| 宿州辣椒地方品种分子遗传多样性分析 |
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| 引用本文： | 高贵珍,赵亮,张兴桃,刘文明,张安文.宿州辣椒地方品种分子遗传多样性分析[J].安徽工程科技学院学报,2013(4):12-14,57. |
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| 作者姓名： | 高贵珍 赵亮 张兴桃 刘文明 张安文 |
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| 作者单位： | [1]宿州学院化学与生命科学学院,安徽宿州234000 [2]宿州市农业科学院,安徽宿州234000 |
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| 基金项目： | 安徽省教育厅重点基金资助项目（KJ2011A262）;宿州学院大学生科研基金资助项目（KYLXLKZD12-05）;国家大学生创新创业训练计划基金资助项目（201210379044） |
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| 摘 要： | 利用优化的辣椒SRAP—PCR反应体系，研究宿州辣椒地方品种的遗传多样性．从30个引物组合中筛选出条带清晰、多态性好的15个引物组合，共扩增出62条谱带．其中，多态性谱带有40条，多态率为64．5％；不同引物扩增的条带为3～7条，谱带大小范围为100～1000bp．供试品种的遗传相似系数为0．52～1．00之间，当遗传距离D=0．52时，可将27份材料分为两大类．结果表明，宿州辣椒种质资源遗传多样性丰富．
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| 关 键 词： | 辣椒 SRAP 遗传多样性 聚类分析 宿州 |
Molecular genetic diversity of local pepper varieties in Suzhou |
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| GAO Gui-zhenI,ZHAO Liang,ZHANG Xing-tao,LIU Wen-ming,ZHANG An-wen.Molecular genetic diversity of local pepper varieties in Suzhou[J].Journal of Anhui University of Technology and Science,2013(4):12-14,57. |
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| Authors: | GAO Gui-zhenI ZHAO Liang ZHANG Xing-tao LIU Wen-ming ZHANG An-wen |
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| Institution: | 2 (1. Dept. of Chem. I Lif. Sci. ,Suzhou College,Suzhou 234000,China 2. Suzhou Agricultural Academy of Sciences, Suzhou 234000,China) |
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| Abstract: | The SRAP molecular marker was used to identify the genetic differences of pepper varieties. On the basis of orthogonally optimized SRAP-PCR amplification system, genetic diversity of 27 pepper varie- ties in Suzhou was studied. 15 effective primer combinations were selected from 30 primer-combinations. A total of 62 bands were detected with those primers and 40 of them were polymorphic. The percentage of polymorphy was 64.5 ~. Their Genetic similitary coeffient ranged from 0.52 to 1.00. Most of the am- plified fragments had a length ranged from 100 to 1000 bp. The cluster analysis using DPS software showed that the tested materials could be categorized into 2 groups at D = 0. 52, the cluster results showed that the genetic differentiation of local pepper varieties in Suzhou was abundant. |
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| Keywords: | pepper SRAP ~ genetic diversity ~ germplasms ~ suzhou |
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