| 增强型绿色荧光蛋白基因真核表达载体的构建 |
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| 引用本文： | 徐洁杰,张伟娟,王浩明,胡火珍.增强型绿色荧光蛋白基因真核表达载体的构建[J].四川大学学报(自然科学版),2005,42(2):386-388. |
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| 作者姓名： | 徐洁杰 张伟娟 王浩明 胡火珍 |
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| 作者单位： | 四川大学生命科学学院细胞生物学教研室,成都,610041;四川大学华西基础医学与法医学院法医物证学教研室,成都,610041;湖北省浠水县人民医院,湖北浠水,438200 |
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| 摘 要： | 构建增强型绿色荧光蛋白(enhanced green fluorescent protein，EGFP)基因的真核表达载体pCDNA3．1( )-EGFP，转染至培养的Hela细胞中成功表达，并发出绿色荧光，证明EGFP一种良好的报告基因和筛选标记．
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| 关 键 词： | 增强型绿色荧光蛋白(EGFP) 真核表达载体 转染 Hela细胞 |
| 文章编号： | 0490-6756(2005)02-0386-03 |
Construction and Expression of Eukaryotic Expression Vector of Enhanced Green Fluorescent Protein Gene |
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| XU Jie-jie,ZHANG Wei-juan,WANG Hao-ming,HU Huo-zhen.Construction and Expression of Eukaryotic Expression Vector of Enhanced Green Fluorescent Protein Gene[J].Journal of Sichuan University (Natural Science Edition),2005,42(2):386-388. |
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| Authors: | XU Jie-jie ZHANG Wei-juan WANG Hao-ming HU Huo-zhen |
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| Institution: | XU Jie-jie1ZHANG Wei-juan2WANG Hao-ming3HU Huo-zhen1 |
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| Abstract: | The eukaryotic expression vector pCDNA3.1(+)-EGFP was constructed for determination of expression of enhanced green fluorescent protein gene in Hela cell. It was demonstrated that EGFP gene was successfully expressed in Hela cells, implying that EGFP was a good reporter and selection marker molecule in mammalian cells. |
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| Keywords: | enhanced green fluorescent protein (EGFP) eukaryotic expression vector transfection Hela cell |
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