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| Polymorphism of human cytomegalovirus （HCMV） UL144 gene in low passage clinical isolates | |
| 引用本文： | Xingxing Fu,;Liping Li,;Chaoyang Huang,;Xiaoman Li,;Huadong Wang,;Zhenyuan Xie,;Ze Chen. Polymorphism of human cytomegalovirus （HCMV） UL144 gene in low passage clinical isolates[J]. 科学通报(英文版), 2014, 59(25): 3120-3126. DOI: 10.1007/s11434-014-0353-z |
| 作者姓名： | Xingxing Fu, Liping Li, Chaoyang Huang, Xiaoman Li, Huadong Wang, Zhenyuan Xie, Ze Chen |
| 作者单位： | [1]Shanghai Institute of Biological Products, Shanghai 200052, China; [2]Institute of Pediatric Research, Hunan Children's Hospital, Changsha 410007, China; [3]Laboratory of Molecular Virology, School of Life Science, Hunan Normal University, Changsha 410084, China; [4]State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China; [5]Xie Tu Community Medical Service Center, Shanghai 200032, China |
| 基金项目： | This work was supported by the Innovation Platform Open Funds for Universities in Hunan Province （09K022 and 11 K010）, the Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province. |
| 摘 要： | To explore the impact of gene polymorphism of human cytomegalovirus （HCMV） on virus virulence, the full-length UL144 gene and partial sequence of glycoprotein B （UL55） gene were sequenced and analyzed for 23 clinical strains of HCMV isolated from urine samples of pediatric patients with congenital or postnatal HCMV infection. Among the 23 isolates, 13 （57 %） were UL144 genotype 1A, 3（13 %） were UL144 genotypes 2 and 7（30 %） were UL144 genotype 3; geographic differences in geno- type distribution were found for both UL144 and gB gene. No UL144 genotypes 1B and 1C were found in this study while these two genotypes were common in HCMV strains isolated in the US. Our results also demonstrated that for all clinical strains of gB genotypes I, III and UL144 genotypes 1A, 2, and 3 found in this study, mother-to-fetus vertical transmission was possible. |
| 关 键 词： | 人巨细胞病毒 基因多态性 HCMV 分离株 临床 GB基因 基因型 糖蛋白B |
Polymorphism of human cytomegalovirus (HCMV) UL144 gene in low passage clinical isolates |
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| Xingxing Fu,Liping Li,Chaoyang Huang,Xiaoman Li,Huadong Wang,Zhenyuan Xie,Ze Chen. Polymorphism of human cytomegalovirus (HCMV) UL144 gene in low passage clinical isolates[J]. Chinese science bulletin, 2014, 59(25): 3120-3126. DOI: 10.1007/s11434-014-0353-z | |
| Authors: | Xingxing Fu Liping Li Chaoyang Huang Xiaoman Li Huadong Wang Zhenyuan Xie Ze Chen |
| Affiliation: | 1. Shanghai Institute of Biological Products, Shanghai, 200052, China 2. Institute of Pediatric Research, Hunan Children’s Hospital, Changsha, 410007, China 3. Laboratory of Molecular Virology, School of Life Science, Hunan Normal University, Changsha, 410084, China 4. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China 5. Xie Tu Community Medical Service Center, Shanghai, 200032, China |
| Abstract: | To explore the impact of gene polymorphism of human cytomegalovirus (HCMV) on virus virulence, the full-length UL144 gene and partial sequence of glycoprotein B (UL55) gene were sequenced and analyzed for 23 clinical strains of HCMV isolated from urine samples of pediatric patients with congenital or postnatal HCMV infection. Among the 23 isolates, 13 (57 %) were UL144 genotype 1A, 3(13 %) were UL144 genotypes 2 and 7(30 %) were UL144 genotype 3; geographic differences in genotype distribution were found for both UL144 and gB gene. No UL144 genotypes 1B and 1C were found in this study while these two genotypes were common in HCMV strains isolated in the US. Our results also demonstrated that for all clinical strains of gB genotypes I, III and UL144 genotypes 1A, 2, and 3 found in this study, mother-to-fetus vertical transmission was possible. |
| Keywords: | Human cytomegalovirus - Genepolymorphism UL144 gene Glycoprotein B （UL55）gene Virus virulence |
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