| 油菜内生真菌的分离鉴定 |
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| 引用本文： | 陈立军,孙广宇,张荣,郭井泉.油菜内生真菌的分离鉴定[J].石河子大学学报,2004,22(Z1):66-68. |
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| 作者姓名： | 陈立军 孙广宇 张荣 郭井泉 |
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| 作者单位： | 陈立军（西北农林科技大学植保学院,陕西,杨凌,712100）
孙广宇（西北农林科技大学植保学院,陕西,杨凌,712100;中国科学院微生物所真菌地衣系统学重点实验室,北京,100080）
张荣（西北农林科技大学植保学院,陕西,杨凌,712100）
郭井泉（拜耳作物科学中国部,北京,100020） |
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| 摘 要： | 本试验通过对健康油菜组织表面消毒、内生真菌的分离培养和鉴定,获得以下结果共获得内生真菌45种, 其中子囊菌1种,半知菌43种(丝孢纲40种,腔孢纲3种),担子菌1种,分离物中37个种分属于20个不同属,8个种归属待定.从分离几率来看,没有明显优势的种类存在,在叶部分出率最高的是Alternaria alternata, 分离几率仅为7.04%.不同油菜器官的内生真菌种类有一定的差异,从叶部分离出25种,根部分离出12种、花器11种、茎12种、幼苗2种.以上结果显示油菜内生真菌复杂、具有丰富的种类多样性.
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| 关 键 词： | 油菜 内生菌 多样性 |
| 文章编号： | 1007-7383(2004)zk-0066-03 |
| 修稿时间： | 2004年4月23日 |
Isolation and Identification of Endophytic Fungi on Brassica Napus |
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| CHEN Li-jun,SUN Guang-yu.Isolation and Identification of Endophytic Fungi on Brassica Napus[J].Journal of Shihezi University(Natural Science),2004,22(Z1):66-68. |
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| Authors: | CHEN Li-jun SUN Guang-yu |
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| Institution: | CHEN Li-jun~1,SUN Guang-yu~ |
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| Abstract: | In this survey,we isolated endophytic fungi from surface-sterilized healthy oilseed rape( Brassica napus ) and identified them.Forty five endophytic fungi were achieved,including 1 Ascomycetces, 1 Basidiomycetes and 43 Fungi Imperfecti(40 in Hyphomycetes and 3 in Coelomycetes ).Thirty seven belonged to 20 genera,and 8 species were unidentified.There were no apparent dominant species. Alternaria alternate from leaf was the highest frequency,7.04%.The species amounts of endophytic fungi isolated from different organs of oilseed rape was variable.We found 25 species from leaves,12 species from roots,11 species from flowers,12 species from stems and 2 species from seedlings.It could be seen from our results that oilseed rape was rich in endophytic fungi. |
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| Keywords: | Brassica napus entophytes diversity |
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