| 温敏性水凝胶作为固定化酶载体的研究 |
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| 引用本文： | 张传梅,付建伟,庄银凤,毛陆原. 温敏性水凝胶作为固定化酶载体的研究[J]. 河南科学, 2006, 24(5): 683-686 |
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| 作者姓名： | 张传梅 付建伟 庄银凤 毛陆原 |
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| 作者单位： | 河南工业大学,化学工业职业学院,郑州,450042;郑州大学,材料工程学院,郑州,450052 |
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| 摘 要： | 提出水凝胶载体材料优化模型,根据优化结果制备出了两种性能相异的载体材料即聚丙烯酰胺/2-甲基丙烯酸羟基乙酯和聚N-异丙基丙烯酰胺/2-甲基丙烯酸羟基乙酯,并用其包埋α-胰凝乳蛋白酶,利用载体材料的温敏性可实现固定化酶活力的可控及连续可调,两种固定化酶经连续催化8次,固定化酶活力并无明显下降.
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| 关 键 词： | 聚丙烯酰胺 聚N-异丙基丙烯酰胺 温敏性水凝胶 固定化酶 |
| 文章编号： | 1004-3918(2006)05-0683-04 |
| 收稿时间： | 2006-04-16 |
| 修稿时间： | 2006-04-16 |
Investigation of Carrier Materials of Immobilized Enzyme Based on Thermosensitive Hydrogel |
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| ZHANG Chuan-mei,FU Jian-wei,ZHUANG Yin-feng,MAO Lu-yuan. Investigation of Carrier Materials of Immobilized Enzyme Based on Thermosensitive Hydrogel[J]. Henan Science, 2006, 24(5): 683-686 |
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| Authors: | ZHANG Chuan-mei FU Jian-wei ZHUANG Yin-feng MAO Lu-yuan |
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| Affiliation: | 1. Vocational College of Chemical Industry, Henan University of Technology, Zhengzhou 450042, China 2. College of Materials Engineering, Zhengzhou University, Zhengzhou 450052, China |
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| Abstract: | In this paper,the optimum model of carrier materials was made and two different immobilized enzymes of α-chymotrypsin based on P(AM-co-HEMA) and P(NIPAM-co-HEMA) were prepared by the optimum results.Immobilized enzyme activity could be controlled and adjusted continuously within a certain range by use of the thermosensitivity of carrier materials and were not reduced obviously after eight times continuously use for the catalyzing reaction. |
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| Keywords: | polyacrylamide polyisopropylacrylamide themosensitive hydrogel immobilized enzyme |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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