| MgSO4对2-酮基-L-古龙酸发酵影响的实验研究 |
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| 引用本文： | 杨涛,朱欣杰,张志雄,吴洪涛,仓基勇,贾茜,袁景淇.MgSO4对2-酮基-L-古龙酸发酵影响的实验研究[J].上海交通大学学报,2008,42(9). |
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| 作者姓名： | 杨涛 朱欣杰 张志雄 吴洪涛 仓基勇 贾茜 袁景淇 |
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| 作者单位： | 1. 上海交通大学自动化系,上海,200240
2. 华北制药集团新药中心,石家庄,050015 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 通过摇瓶实验和2 L搅拌釜式发酵罐实验,研究了发酵培养基中初始MgSO4浓度对维生素C前体2-酮基-L-古龙酸(2-KGA)发酵过程的影响.实验结果表明:2-KGA发酵的最佳MgSO4初始浓度w0≈0.084%,在这一浓度下,产物2-KGA的得率和容积生产率均达到峰值;MgSO4初始浓度过高将降低产物得率和容积生产率.
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| 关 键 词： | 2-酮基-L-古龙酸 硫酸镁 得率 容积生产率 |
The Effect of MgSO4 on 2-Keto-L-Gulonic Acid Fermentation |
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| YANG Tao,ZHU Xin-jie,ZHANG Zhi-xiong,WU Hong-tao,CANG Ji-yong,JIA Qian,YUAN Jing-qi.The Effect of MgSO4 on 2-Keto-L-Gulonic Acid Fermentation[J].Journal of Shanghai Jiaotong University,2008,42(9). |
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| Authors: | YANG Tao ZHU Xin-jie ZHANG Zhi-xiong WU Hong-tao CANG Ji-yong JIA Qian YUAN Jing-qi |
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| Abstract: | The influences of MgSO_4 initial concentration on the 2-keto-L-gulonic acid(2-KGA) fermentation were studied through shaking flasks culture as well as fermentation in 2-L jars.The experimental results indicate that 0.084% is the best MgSO_4 initial concentration for the 2-KGA fermentation process.With such MgSO_4 concentration,both the conversion ratio of L-sorbose and the 2-KGA production rate are found to be the highest.The experiments also show that with MgSO_4 concentration higher than 0.084%,both 2-KGA yield and volumetric productivity will be decreased. |
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| Keywords: | 2-keto-L-gulonic acid(2-KGA) MgSO_4 yield volumetric productivity |
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