低温脂肪酶产生菌的筛选及鉴定

通过三丁酸甘油酯平板法从太平洋帕里西维拉海盆5010m的底泥中共筛出八株可产脂肪酶的菌株，其中的两株生理生化特征极其相近的菌脂肪酶活性最高，并且对橄榄油平板产生荧光。对两株菌进行鉴定，分析其生理生化特征和16SrDNA产物序列，确定这两株菌均属于发光杆菌属，但是和该属的各种还有一定差异，对其分类地位的最后确定还需进一步的系统发育学分析。其中D2菌株所产脂肪酶的最适作用温度在35℃左右，证明其脂肪酶为低温酶；最适作用pH值在7～9之间，最适pH值8。

Screening and classification of cold-adapted lipase-producing bacterium and part characterization of the lipase

Eight lipase-producing bacterium strains were screened from 40 cold-adapted strains, these strains isolated from the mud of Parece Vela sea basin in the Pacific Ocean of 5010m deep, of which two similar strains have the highest lipolytic activity and are positive to olive oil. Through the traditional physiological and biochemical method, together with the 16SrDNA sequence homology and phylogenetic analysis, the strains were identified. The result shows they belong to the genus photobacterium, but they differ from any species of this genus in some characteristic which suggests they might be new species, and their taxonomic placement needs more information on genes level. The optimal temperature of the action of D2 crude lipases is about 35℃ which supports that the lipase is a cold-adapted one, and its optimal pH for activity is 8.