自噬对肝癌SMMC-7721细胞侵袭迁移能力的影响

为探讨自噬对照射过程中肝癌SMMC-7721细胞侵袭和迁移能力的影响及其潜在的作用机制，将肝癌SMMC-7721细胞分为6组，分别为阴性对照(NC)组、8 Gy X-线照射(IR)组、自噬激活剂雷帕霉素(Rapa)组、自噬抑制剂三甲基腺嘌呤(3-MA)组、照射+雷帕霉素(IR+Rapa)组和照射+三甲基腺嘌呤(IR+3-MA)组，利用划痕实验和Transwell实验检测自噬对肝癌SMMC-7721细胞迁移和侵袭的影响；用CCK8实验检测照射、雷帕霉素和3-MA对肝癌细胞增殖的影响；用蛋白免疫印迹(Western blot)检测N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)和LC3B蛋白表达情况。研究结果显示，照射可增强肝癌SMMC-7721细胞侵袭迁移的能力(P<0.05),同时，照射可抑制细胞的增殖能力(P<0.05);雷帕霉素激活自噬可增强细胞的侵袭迁移能力(P<0.05),3-MA抑制自噬可抑制细胞侵袭迁移和细胞的增殖能力(P<0.05)。照射可上调N-cadherin、Vimentin表达水平(P<0.05),激活或抑制自噬可分...

Effect of Autophagy on Invasion and Migration of Hepatocellular Carcinoma SMMC-7721 Cells

To investigate the effect of autophagy on the invasion and migration of Hepatocellular Carcinoma (HCC) SMMC-7721 cells during irradiation and its potential mechanism,the HCC SMMC-7721 cells were divided into 6 groups,namely negative control (NC) group,8 Gy X-ray irradiation (IR) group,autophagy activator rapamycin (Rapa) group,autophagy inhibitor trimethyl Adenine (3-MA) group,irradiation+rapamycin (IR+Rapa) group,irradiation+trimethyladenine (IR+3-MA) group.The effects of autophagy on the migration and invasion of HCC SMMC-7721 cells were detected by scratch assay and Transwell assay.CCK8 assay was used to detect the effects of irradiation,rapamycin and 3-MA on the proliferation of HCC cells.The protein expressions of N-cadherin,Vimentin and LC3B were detected by Western blot.The results showed that irradiation could enhance the ability of invasion and migration of SMMC-7721 cells (P<0.05),and at the same time,irradiation could inhibit the proliferation of cells (P<0.005).Rapamycin activated autophagy could promote the ability of cell invasion and metastasis (P<0.05),and 3-MA inhibited autophagy could inhibit the ability of cell invasion,migration and cell proliferation (P<0.05).Irradiation could up-regulate the protein expression levels of N-cadherin and Vimentin (P<0.05),and activation or inhibition of autophagy could increase or inhibit the protein expression levels of N-cadherin and Vimentin (P<0.05).It indicated that X-ray irradiation could activate autophagy and promote the occurrence of Epithelial-Mesenchymal Transition (EMT).Activation of autophagy could improve epithelial-mesenchymal transition of HCC cells and promote invasion and migration of HCC cells.On the contrary,inhibition of autophagy could hinder the invasion and migration of HCC cells.