| 猪GPR84基因克隆和组织表达图谱分析 |
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| 引用本文： | 徐雪,张剑南,张耀荣,王亚军,李娟. 猪GPR84基因克隆和组织表达图谱分析[J]. 四川大学学报(自然科学版), 2014, 51(5): 1079-1084 |
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| 作者姓名： | 徐雪 张剑南 张耀荣 王亚军 李娟 |
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| 作者单位： | 四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都,610065 |
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| 基金项目： | 国家重大专项转基因生物新品种培育(2009zx08009 145B) |
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| 摘 要： | 本研究以长白猪(Landrace)小肠cDNA为模板,克隆游离脂肪酸家族受体GPR84基因cDNA全长.GPR84基因cDNA全长1243bp,编码396个氨基酸的前体蛋白,与人、大鼠、小鼠、牛等哺乳动物的GPR84氨基酸序列一致性分别为90.2%、83.8%、82.8%、89.4%;结构分析表明猪GPR84含有保守的七次跨膜结构域.采用RT-PCR方法,对家猪GPR84基因进行组织表达分析.结果显示:GPR84在家猪各组织中均有表达.其在下丘脑,肺,肝脏及肠末端分区等组织中表达量较高.
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| 关 键 词： | 家猪 G蛋白偶联受体84 分子克隆 组织表达 |
| 收稿时间： | 2014-04-17 |
Molecular cloning,sequence analysis and tissue expression of G protein-coupled receptor 84 (GPR84) in pigs (Sus scrofa) |
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| XU Xue , ZHANG Jian-Nan , ZHANG Yao-Rong , WANG Ya-Jun , LI Juan. Molecular cloning,sequence analysis and tissue expression of G protein-coupled receptor 84 (GPR84) in pigs (Sus scrofa)[J]. Journal of Sichuan University (Natural Science Edition), 2014, 51(5): 1079-1084 |
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| Authors: | XU Xue ZHANG Jian-Nan ZHANG Yao-Rong WANG Ya-Jun LI Juan |
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| Affiliation: | XU Xue;ZHANG Jian-Nan;ZHANG Yao-Rong;WANG Ya-Jun;LI Juan;Key laboratory of Bio-resources and Eco-environment of Ministry of Education,college of Life Sciences,Sichuan University; |
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| Abstract: | The full length cDNAs of pig GPR84 (pGPR84) gene were cloned from pig brain tissue using RT PCR. The cloned pGPR84 was 1243bp in length and encodes a protein of 396 amino acids, which shares high amino acid sequence identity with that of human (90.2 %), mouse (83.8 %), rat ( 82.8%) and cattle (89.4%). Structural analyses indicated that pGPR84 containsed a conversed 7 transmembrane domain. RT PCR assay showed that pGPR84 was abundantly expressed in hypothalamus, liver, kidney and intestine and weakly in brain, heart, spleen and muscle. |
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| Keywords: | Pig G protein coupled receptor 84 Molecular cloning Tissue distribution |
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