| 大鼠肌肉素cDNA克隆及表达 |
| |
| 引用本文： | 裘梁,王曼莹,唐文琴,廖志勇,张霆,陈晓丽.大鼠肌肉素cDNA克隆及表达[J].江西师范大学学报(自然科学版),2006,30(4):387-391. |
| |
| 作者姓名： | 裘梁 王曼莹 唐文琴 廖志勇 张霆 陈晓丽 |
| |
| 作者单位： | 1. 江西师范大学,生命科学学院,南昌,江西,330022
2. 首都儿科研究所,北京,100020 |
| |
| 摘 要： | 通过RT-PCR方法从大鼠骨骼肌中克隆到肌肉素cDNA,构建表达载体pGEX-5X-3-musclin,并在BL21大肠杆菌中成功表达了融合蛋白GST-Musclin,且对表达条件进行了优化.在最优化的表达条件下,融合蛋白的表达量达到了14.2%.
|
| 关 键 词： | 肌肉素 克隆 表达 |
| 文章编号： | 1000-5862（2006）04-0387-05 |
| 收稿时间： | 2006-01-12 |
| 修稿时间： | 2006-01-12 |
Rat Musclin: Cloning and Expression in Escherichia Coli |
| |
| QIU Liang,WANG Man-ying,TANG Wen-qing,LIAO Zhi-yong,ZHANG Ting,CHEN Xiao-li.Rat Musclin: Cloning and Expression in Escherichia Coli[J].Journal of Jiangxi Normal University (Natural Sciences Edition),2006,30(4):387-391. |
| |
| Authors: | QIU Liang WANG Man-ying TANG Wen-qing LIAO Zhi-yong ZHANG Ting CHEN Xiao-li |
| |
| Institution: | 1. Department of Life Science, Jiangxi Normal University, Nanchang 330027, China; 2. Paediatric Institute of Capital, Beijing 100020, China |
| |
| Abstract: | In this paper,musclin cDNA was cloned from skeletal muscle of rat by RT-PCR.Then the gene fragment was cloned into expression vector pGEX-5x-3,and the recombinant was transformed into E coli BL21.Induced by IPTG,the fusion protein GST-musclin,a 40 kDa protein was expressed in E coli BL21.and identified in SDS-PAGE In the optimum conditions,the expression level of fusion protein was 14.2%. |
| |
| Keywords: | musclin cloning expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
