| 负载丝裂霉素C的聚乳酸微球制备及细胞生长抑制作用研究 |
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| 引用本文： | 朱继翔,彭晔,田秀梅,阳范文,陈晓明.负载丝裂霉素C的聚乳酸微球制备及细胞生长抑制作用研究[J].华南师范大学学报(自然科学版),2014,46(1):65-70. |
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| 作者姓名： | 朱继翔 彭晔 田秀梅 阳范文 陈晓明 |
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| 作者单位： | 1.1.广州医科大学基础学院生物医学工程系 |
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| 基金项目： | 国家自然科学基金;广东省重大科技专项 |
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| 摘 要： | 利用单乳化溶剂挥发法制备负载丝裂霉素C（MMC）的聚乳酸（PLA）载药微球. 优化载药微球的制备条件，当药物与载体聚合物比例为10:90时，微球的实际载药量与包封率分别达到最高值5.62%与49.1%；采用SEM对微球形貌进行表征；对载药微球的体外释药进行研究，结果表明载药微球无明显暴释现象，可有效缓释MMC达30天以上，累计释放量为84.8%；细胞实验结果表明，载药微球可以有效抑制小鼠NIH-3T3成纤维细胞的增殖.
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| 关 键 词： | 控制释放 |
| 收稿时间： | 2013-07-06 |
Fabrication of mitomycin C loaded polylactic acid microsphere and inhibition study of cell |
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| Zhu Jixiang;Peng Ye;Tian Xiumei;Yang Fanwen;Chen Xiaoming.Fabrication of mitomycin C loaded polylactic acid microsphere and inhibition study of cell[J].Journal of South China Normal University(Natural Science Edition),2014,46(1):65-70. |
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| Authors: | Zhu Jixiang;Peng Ye;Tian Xiumei;Yang Fanwen;Chen Xiaoming |
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| Institution: | Zhu Jixiang;Peng Ye;Tian Xiumei;Yang Fanwen;Chen Xiaoming;Department of Biomedical Engineering,Guangzhou Medical University; |
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| Abstract: | Mitomycin C (MMC) loaded polylactic acid (PLA) microspheres were fabricated by oil-in-water (O/W) single-emulsion solvent evaporation technique. The preparation conditions were optimized. The results indicated that the drug loading rate and encapsulation rate reached maximal values (5.62% and 49.1%) when the ratio of MMC to PLA was 10/90. The morphology of microspheres was observed by scanning electron microscopy. The release tests showed that the microspheres could control release MMC over 30 days and the cumulative release was 84.8% in vitro. Microspheres were co-cultured with mouse NIH-3T3 fibroblast cells and the MTT results showed that the MMC loaded microspheres could effectually inhibit the cell growth. |
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