水稻光敏色素A的克隆及其在光形态建成中的作用

克隆了OsPHYA基因，分析光暗诱导下该基因的表达、极低辐照度反应（VLFR）条件下萌发率测定、VLFR和高辐照度反应（HIR）条件下胚芽鞘伸长以及白化苗转绿过程中叶绿素含量测定等实验. 结果表明，OsPHYA基因在黑暗条件下根、茎和叶中的积累水平高于光照处理；OsPHYA在VLFR下能促进水稻种子萌发；而在VLFR和HIR反应中，OsPHYA抑制水稻胚芽鞘的伸长.对OsphyA突变体和野生型的主要农艺性状进行了比较分析，发现2种基因型仅在每株穗数方面有一定差异，说明光敏色素A对水稻农艺性状的影响不明显.

Cloning and Function Analysis of Rice PhytochromeA in Photomorphogenesis

Phytochrome is the first discovered phytochrome in higher plants, which is Phytochrome is the first discovered phytochrome in higher plants, which is primarily involved in far-red light sensing (high irradiance response, HIR) and in responses to a pulse of very weak light (very low fluence response, VLFR). In order to investigate the functions of OsPHYA in photomorphogenesis, we cloned OsPHYA gene and did a series of experiments including expression analysis of OsPHYA gene in different organs under light and dark, seed germination rate under VLFR, coleoptile length under VLFR and HIR, and chlorophyll content in process of etiolated seedling turning green. The results showed that expression level of OsPHYA gene in root, stem and leaf under dark was higher than that under light, OsPHYA could promote rice seed germination under VLFR, OsPHYA inhibited coleoptile elongation in responses to VLFR and HIR. We further detected agronomic characters between the OsphyA mutants and WT seedlings, which showed there was no significant differences except for panicle number per plant, indicating that OsphyA has no obvious effect on agronomic characters in rice