龙眼不同愈伤组织培养方法的探讨及细胞观察

试验中以龙眼"红核子"品种胚性愈伤组织LC2细胞系为材料,研究不同条件对龙眼胚性愈伤组织生长的影响以及龙眼非胚性愈伤组织培养体系的建立,并通过显微镜观察比较龙眼胚性愈伤组织和非胚性愈伤组织的形态学差异。试验结果表明,高9cm、直径6cm、瓶口内径5cm的玻璃培养瓶在接种量约为30mg/瓶时龙眼胚性愈伤组织生长最好;龙眼非胚性愈伤组织以MS+6-BA 1.0 mg/L+2,4-D 0.5 mg/L+活性炭0.5 mg/L培养效果最好;细胞观察表明,龙眼胚性愈伤组织较非胚性愈伤组织细胞更规则,有更多的内含物。

Study onCultivating Methods and Observation of Different Callus in Longan

In this experiment, longan cultivar ＇ Honghezi＇ LC2 emhryogenic callus was used as the materials to investigate the effects of different cultivating methods on the growth of callus, and the culture system of non--emhryogenic callus in longan was built. In addition, we observed the morphological differences between embryogenic and non- embryogenic callus in longan by microscope. The results showed that longan embryogenic callus grew best in glass vessels with 9 cm height, 6 cm outer diameter and 5 cm inner diameter when inoculum density was 30 mg/bottle. The medium formula of MS＋ 6- BA 1.0 mg/L＋ 2, 4- D 0.5 mg/L＋ AC 0.5 mg/L was most beneficial to cultivating longan non-- embryogenic callus. In addition, we found that longan embryogenic callus was more regular in morphology and had more cell inclusion than longan non--embryogenic callus.