微波和足叶乙苷体外诱导K562细胞凋亡的研究

探讨微波和足叶乙苷体外诱导Ｋ５６２细胞凋亡的可能性及其机制，将微波和足叶乙苷分别单独以及联合作用于下沉髓细胞和Ｋ５６２细胞株，通过细胞凋亡和ｂｃｌ－２蛋白阳性率的测试来评价细胞凋良的程度并研究其机制，微波和足叶乙苷各自都能诱导正常骨髓细胞和Ｋ５６２细胞的少量凋亡，两者联合作用所诱导的细胞凋亡率较之宇足叶乙苷有显著性增加，其中Ｋ５６２的细胞凋亡率的增加幅度远大于正常骨髓细胞，细胞凋亡率的增另和ｂｃｌ

STUDIES ON THE COMBINATION OF MICROWAVE AND ETOPOSIDE FOR INDUCING K562 CELL APOPTOSIS IN VITRO

To study the possibility and the mechanism of K562 apoptosis induced by microwave and etoposide. Methods: Normal human bone marrow cell and K562 cell line were treated by microwave and etoposide alone or by the combination of both. The ratio of apoptotic cell(RAC) and the ratio of bcl_2 protein positive cell(Rbcl_2PC) were measured in order to evaluate cell apoptosis and to study its mechanism. Results: Both microwave and etoposide could induce a little apoptosis of these two kinds of cells,while the combination of microwave and etoposide could induce a remarkable higher RAC than etoposide could do. ( P <0.05)At the same time, RAC for K562 increased much more higher than that for normal human bone marrow cell. RAC increased simultaneously with Rbcl-2PC reduction. Conclusion: The combination of microwave and etoposide showed a limited synergistic effect on inducing K562 apoptosis, and this effect was related to Rbcl-2PC reduction.