| Molecular characterization of human reticulon 3 as a potential marker during the differentiation of human neuroblastoma SH-SY5Y cells |
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| 作者姓名： | YANG Hongbo HUANG Xiaowei YIN Bin PENG Xiaozhong FAN Ming QIANG Boqin C |
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| 作者单位： | National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China,National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China,National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China,National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China,Department of Neurobiology, Institute of Basic Medical Science, Academy of Military Medical Sciences, Beijing 100850, China） ,National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China,National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，国家高技术研究发展计划(863计划)，国家自然科学基金 |
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| 摘 要： | Neuroendocrine-specific protein (NSP) -reticulons are endoplasmic reticulum-associated protein complexes, which are localized in the endoplasmic reticulum (ER) and identified as markers for neuroendocrine differentiation. In the present study, human reticulon 3 gene (hRTN3) was cloned and its expression pattern in a variety of tissues was investigated. Truncated hRTN3s corresponding to the C-terminal (hRTN3-C) domain were expressed and purified. hRTN3 mRNA was down-regulated during the differentiation of human neuroblastoma cell line SH-SY5Y induced by all-trans-retinoic acid (RA), which suggests that, like other members of the reticulon family, hRTN3 is a potential marker for neuroendocrine differentiation.
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| 关 键 词： | human reticulon 3 expression differentiation |
Molecular characterization of human reticulon 3 as a potential marker during the differentiation of human neuroblastoma SH-SY5Y cells |
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| YANG Hongbo,HUANG Xiaowei,YIN Bin,PENG Xiaozhong,FAN Ming,QIANG Boqin,C.Molecular characterization of human reticulon 3 as a potential marker during the differentiation of human neuroblastoma SH-SY5Y cells[J].Progress in Natural Science,2004,14(7):642-645. |
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| Authors: | Yang Hongbo Huang Xiaowei YIN Bin PENG Xiaozhong FAN Ming FAN Wenhong QIANG Boqin C |
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| Abstract: | Neuroendocrine-specific protein (NSP) -reticulons are endoplasmic reticulum-associated protein complexes, which are localized in the endoplasmic reticulum (ER) and identified as markers for neuroendocrine differentiation. In the present study, human reticulon 3 gene (hRTN3) was cloned and its expression pattern in a variety of tissues was investigated. Truncated hRTN3s corresponding to the C-terminal (hRTN3-C) domain were expressed and purified. hRTN3 mRNA was down-regulated during the differentiation of human neuroblastoma cell line SH-SY5Y induced by all-trans-retinoic acid (RA), which suggests that, like other members of the reticulon family, hRTN3 is a potential marker for neuroendocrine differentiation. |
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| Keywords: | human reticulon 3 expression differentiation |
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