Using laser confocal scanning microscope to study ischemia-hypoxia injury in rat brain slice |
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| Authors: | Xiaoying Wang Hong Xing Qihua He Jialing Xu Benjie Wu |
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| Institution: | (1) Department of Biophysics, Beijing Medical University, 100083 Beijing, China;(2) Analytic Center of Medico-Pharmacy, Beijing Medical University, 100083 Beijing, China |
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| Abstract: | The level of lipid peroxidation and cellular necrosis in rat living brain slices during brain ischemia-hypoxia injury have
been observed using a laser confocal scanning microscope (LCSM) with double labeling of fluorescent probes D-399 (2, 7-dichlorofluorescin
diacetate) and propidium iodide (PI). The hypoxia and/or reoxygenation injury in rat brain slices is markedly decreased by
pretreatment with L-NG-nitro-arginine (L-NNA) and N-acetylcysteine (NAC), showing that the nitric oxide (NO) and other free
radicals play an important role in brain ischemia-hypoxia injury. |
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| Keywords: | laser confocal scanning microscope brain ischemia-hypoxia injury lipid peroxide 2 7-dichlorofluorescin diacetate |
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