| 管囊酵母的诱变育种及甜高粱渣水解液发酵 |
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| 引用本文： | 赵磊,张栩,谭天伟.管囊酵母的诱变育种及甜高粱渣水解液发酵[J].北京化工大学学报(自然科学版),2009,36(2):73-76. |
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| 作者姓名： | 赵磊 张栩 谭天伟 |
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| 作者单位： | 北京化工大学,北京市生物加工过程重点实验室,北京,100029;北京化工大学,北京市生物加工过程重点实验室,北京,100029;北京化工大学,北京市生物加工过程重点实验室,北京,100029 |
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| 摘 要： | 对管囊酵母1771进行硫酸二乙酯(DES)与紫外线(UV)复合诱变，筛选得到一株菌DU-13，其糖醇转化率较出发菌株提高28.7%。用DU-13菌株在甜高粱渣酸水解液和发酵培养基中进行发酵，乙醇产量分别为3.5g/L和7.4g/L；转化率分别为0.173g/g和0.250g/g，水解液发酵效果不如发酵培养基。在甜高粱渣酸水解液中添加营养物质，并设计正交实验优化添加方案。将优化水解液在相同条件下利用DU-13菌株进行发酵，结果表明优化水解液发酵效果优于发酵培养基和水解液：乙醇产量为13.5g/L，转化率为0.378g/g。
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| 关 键 词： | 管囊酵母 复合诱变 甜高粱渣水解液 乙醇发酵 |
| 收稿时间： | 2008-04-18 |
The effect of mutation of Pachysolen tannophilus with diethyl sulfate (DES)+UV irradiation on the ethanol fermentation of sweet sorghum residue hydrolysate |
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| ZHAO Lei,ZHANG Xu,TAN TianWei.The effect of mutation of Pachysolen tannophilus with diethyl sulfate (DES)+UV irradiation on the ethanol fermentation of sweet sorghum residue hydrolysate[J].Journal of Beijing University of Chemical Technology,2009,36(2):73-76. |
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| Authors: | ZHAO Lei ZHANG Xu TAN TianWei |
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| Institution: | Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China |
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| Abstract: | Diethyl sulfate(DES)+UV mutation was carried on Pachysolen tannophilus 1771 and the strain DU-13 was obtained;mutation increased the ethanol yield by 28.7%.Fermentation of sweet sorghum residue hydrolysate and of fermentation medium by P.tannophilus were performed.Hydrolysate fermentation afforded a lower ethanol concentration of 3.5 g/L and ethanol yield of 0.173 g/g compared with the corresponding values of 7.4 g/L and 0.250 g/g obtained for the fermentation medium.Fermentation of the hydrolysate medium w... |
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| Keywords: | Pachysolen tannophilus complex mutagenesis sweet sorghum residue hydrolysate ethanol fermentation |
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