金属离子对荧光单假胞菌P13生长及抗真菌素合成的影响

以油菜菌核病菌为供试病原菌，探讨11种金属离子对荧光假单胞菌P13的生长及拮抗能力的影响．结果表明，金属离子均对P13的生物量以及抗生素的合成产生一定的影响．与对照相比，金属离子在0．002mol／L的浓度下，Ag^＋，Cr^3＋，Ni^＋能强烈抑制该菌的生长；Ca^2＋，Ti^3＋，Fe^3＋有微弱的促生作用；Al^3＋能强烈抑制P13抗生素的合成，Ca^2＋和Fe^3＋则明显促进抗生素的合成．在不同浓度的Ca^2＋和Fe^3＋下，0．003mol／L的Ca^2＋促进P13的相对效价提高到267％．0．003mol／L的Ca^2＋与0．001mol／L Fe^3＋共同使用时P13的效价增加到291％，说明Ca^2＋和Fe^3＋对P13抗真菌素合成有协同作用．

Effect of metal ions on cell growth and antibiotic production of Pseudomonas fluorescens P13

The effect of eleven different metal ions on cell growth and control on Sclerotinia sclerotiorum of Pseudomonas fluorescens P13 were studied.The results showed that the metal ions certainly affect the cell growth and production of antibiotics of P13.At the concentration of 0.0002mol/L,contrasting to the comparison,Ag+,Cr3+,Ni+ can restrain cell growth intensively;Ca2+,Ti3+,Fe3+can boost cell growth faintly;Al3+ can reduce the production of antibiotic sorely;Ca2+,Fe3+ can add the production of antibiotic distinctly.With different concentration of Ca2+,Fe3+,we found that Ca2+(0.003mol/L),Ca2+(0.003mol/L) and Fe3+(0.001mol/L)mixed all promote the production of antibiotic of P13.It concluded that Ca2+ and Fe3+ cooperated to add the production of antibiotic in P13.