Administration of D-alanine did not cause increase of D-amino acid oxidase activity in mice |
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Authors: | Y. Nagata R. Yamada H. Nagasaki R. Konno Y. Yasumura |
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Affiliation: | (1) Laboratory of Biology, Himeji Institute of Technology, Shosha 2167, 671-22 Himeji, (Japan);(2) Department of Bioengineering, Nagaoka University of Technology, 940-21 Nagaoka, (Japan);(3) Department of Biochemistry, Kyoto Prefectural University of Medicine, 602 Kyoto, (Japan);(4) Department of Microbiology, Dokkyo University School of Medicine, 321-02 Tochigi, (Japan) |
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Abstract: | D-amino acid oxidase (DAAO) activity was not altered in the liver and kidney by oral administration of D-alanine to adult mice. The enzyme was apparently not induced by the enteric microflora either, since the enzyme activity in the liver and kidney of germ-free mice was not different from that of specific-pathogen-free mice. The times of appearance of DAAO activity and of free D-amino acids in the kidney were elucidated using suckling mice. DAAO activity started to increase 7 days after birth, and reached almost the adult level by 28 days. The content of free neutral D-amino acids also increased with age, in a similar fashion. A possible conclusion is that the enzyme activity normally increases during this period, to eliminate the free D-amino acids which have increased with age in the suckling mice. Consequently, the administration of D-alanine had no further effect in increasing enzyme activity. |
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Keywords: | D-amino acid oxidase D-amino acids D-alanine microflora induction germ free |
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