| 单倍体小麦与簇毛麦的不对称体细胞杂交 |
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| 引用本文： | 周爱芬,向凤宁,夏光敏,陈惠民,黄粤,翟晓灵. 单倍体小麦与簇毛麦的不对称体细胞杂交[J]. 山东大学学报(理学版), 1999, 34(2): 224-229 |
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| 作者姓名： | 周爱芬 向凤宁 夏光敏 陈惠民 黄粤 翟晓灵 |
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| 作者单位： | 1. 山东大学生命科学学院
2. 青岛市农业科学研究所 |
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| 摘 要： | 以小麦杂２４２（Ｔｒｉｔｉｃｕｍａｅｓｔｉｖｕｍ，ｃｖ．２４２）花药来源的愈伤组织分离原生质体作受体；簇毛麦（Ｈａｙｎａｌｄｉａｖｉｌｏｓａ）幼胚来源的愈伤组织原生质体经２２０μＷ／ｃｍ２紫外线照射３０ｓ作供体，用ＰＥＧ法诱导融合，最后获得再生愈伤组织．对融合再生的４个最先长大的细胞系进行染色体，同工酶和ＲＡＰＤ分析．结果表明，这些细胞系均为体细胞杂种，ＲＡＰＤ技术可作为小麦体细胞杂种早期鉴定的有效方法
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| 关 键 词： | 单倍体小麦 簇毛麦 不对称体细胞杂交 RAPD分析 |
| 修稿时间： | 1998-02-17 |
ASYMMETRIC SOMATIC HYBRIDIZATION BETWEEN HAPLOID WHEAT AND DIPLOID HAYNALDIA VILLOSA |
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| Zhou Aifen,Xia Guangmin,Xiang Fengning,Chen Huimin,Huang Yue,Zhai Xiaoling. ASYMMETRIC SOMATIC HYBRIDIZATION BETWEEN HAPLOID WHEAT AND DIPLOID HAYNALDIA VILLOSA[J]. Journal of Shandong University, 1999, 34(2): 224-229 |
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| Authors: | Zhou Aifen Xia Guangmin Xiang Fengning Chen Huimin Huang Yue Zhai Xiaoling |
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| Abstract: | Protoplasts isolated from anther derived calli of common wheat ( Triticum aestivum ,cv 24 2) were fused with UV treated protoplasts isolated from young embryo derived calli of Haynaldia villosa by PEG method.The regenerated calli were verified by morphological inspection,chromosome counting,isozyme analysis and RAPD analysis.The results confumed that 4 cell clones formed early were somatic hybrids and RAPD approaching is proposed as a convenient toll for early identification of fusion products. |
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| Keywords: | haploid wheat haynaldia villosa asymmetric somatic hybridization ultroviolet radiation RAPD analysis |
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