| ptr-MIR156a启动子克隆及特征分析 |
| |
| 引用本文： | 陈英,王浩然,许庆,黄敏仁.ptr-MIR156a启动子克隆及特征分析[J].南京林业大学学报(自然科学版),2011,35(6):1-5. |
| |
| 作者姓名： | 陈英 王浩然 许庆 黄敏仁 |
| |
| 作者单位： | 南京林业大学杨树种质创新与品种改良重点实验室,江苏 南京,210037 |
| |
| 基金项目： | 国家林业局林业公益性行业科研专项项目(200904048) |
| |
| 摘 要： | miR156a在火炬松、烟草、拟南芥中的表达与病原菌侵染密切相关。为了研究miR156a在杨树与病原菌互作过程中分子机制,以毛果杨全基因组DNA为材料,预测ptr-MIR156a启动子的大概区域,设计特异性PCR引物,克隆了ptr-MIR156a上游启动子区500、1 000和1 500 bp片段,并进行顺式作用元件分析,然后分别构建了绿色荧光蛋白(GFP)报告基因植物表达载体,最后通过原生质体的瞬时表达体系对其进行了活性检测。结果表明,1 500 bp片段活性最高,1 000 bp片段次之,500 bp片段活性最低。
|
| 关 键 词： | 毛果杨 miR156a 启动子 克隆 特性分析 |
Clone and characterization of ptr-MIR156a promoter in Populus trichocarpa |
| |
| CHEN Ying,WANG Haoran,XU Qing,HUANG Minren.Clone and characterization of ptr-MIR156a promoter in Populus trichocarpa[J].Journal of Nanjing Forestry University(Natural Sciences ),2011,35(6):1-5. |
| |
| Authors: | CHEN Ying WANG Haoran XU Qing HUANG Minren |
| |
| Institution: | CHEN Ying,WANG Haoran,XU Qing,HUANG Minren*(Poplar Germplasm Enhancement & Variety Improvement Lab,Nanjing Forestry University,Nanjing 210037,China) |
| |
| Abstract: | MicroRNAs(miRNAs) are endogenous small RNAs that have large regulatory effects development and stress responses in plants.Recently studies show that the expression of miR156a is intensively related with pathogens infection in Loblolly pine,tobacco,Arabidopsis and maize.To understand the interaction mechanism between miR156a and poplar,we predicted ptr-MIR156a promoter region and amplified three upstreaming fragments of ptr-MIR156a from Populus trichocarpa,500 bp,1 000 bp,and 1 500 bp,respectively,analyzed t... |
| |
| Keywords: | Populus trichocarpa miR156a promoter clone characterization |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
