| 肝癌细胞株HepG-2的G1期细胞蛋白质组双向电泳图谱的建立 |
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| 引用本文： | 白瑞霞,王文礼,李丽梅,刘婷,叶纪诚. 肝癌细胞株HepG-2的G1期细胞蛋白质组双向电泳图谱的建立[J]. 科学技术与工程, 2008, 8(1): 161-163 |
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| 作者姓名： | 白瑞霞 王文礼 李丽梅 刘婷 叶纪诚 |
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| 作者单位： | 内蒙古医学院生物化学与分子生物学教研室,呼和浩特,010059 |
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| 基金项目： | 内蒙古医学院校科研和教改项目 |
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| 摘 要： | 采用双向电泳分离肝癌细胞株 HepG2 的 G1 期细胞的蛋白质,经考马斯亮蓝染色及图像扫描与分析显示:在 pH3-10,13 cm 的 IPG 胶条上,可分离到 227 个重复性及分辨率均较好的蛋白斑点,蛋白斑点的匹配率为 84%.建立了 HepG2 的G1 期细胞蛋白质组双向电泳图谱,为其蛋白质组学的进一步研究奠定了基础.
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| 关 键 词： | 双向电泳 HepG2细胞 蛋白质组 |
| 文章编号： | 1671-1819(2008)1-0161-03 |
| 收稿时间： | 2007-09-21 |
| 修稿时间： | 2007-09-21 |
Development of Two-dimensional Gel Electrophoresis Profiles of Proteome from G1 Phase of HepG2 Cells |
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| BAI Rui-xi,WANG Wen-li,LI Li-mei,LIU Ting,YE Ji-cheng. Development of Two-dimensional Gel Electrophoresis Profiles of Proteome from G1 Phase of HepG2 Cells[J]. Science Technology and Engineering, 2008, 8(1): 161-163 |
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| Authors: | BAI Rui-xi WANG Wen-li LI Li-mei LIU Ting YE Ji-cheng |
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| Abstract: | G1 phase of HepG2 cell proteome was separated by 2-D PAGE.After being stained by Coomassie Brilliant Blue then followed by image scanning and analyzsis,227 protein spots of good repeatability and resolution were detected in the 2-DE profiles on 13 cm IPG strip(pH 3-10)of G1 phase of HepG2 cell.A match rate of 84% of the spots between gels was obtained.The 2-DE profiles of proteome from G1 phase of HepG2 cells is established and lay foundations for the proteome research. |
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| Keywords: | two-dimensional gel electrophoresis G1 phase of HepG2 cell line proteome |
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