Identification of antiviral-relevant genes in the cultured fish cells induced by UV-inactivated virus |
| |
Authors: | Yibing?Zhang Qiya?Zhang Dequan?Xu Chengyu?Hu Email author" target="_blank">Jianfang?GuiEmail author |
| |
Institution: | (1) State Key Laboratory of Freshwater Ecology and Biotechnology, Wuhan Center for Developmental Biology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072 Wuhan, China |
| |
Abstract: | UV-inactivated grass carp hemorrhage virus (GCHV) can induce high titer of interferon in cultured CAB (crucian carp (Carassius auratus L.) blastulae) cells, and thus defend host cells against the virus invasion. The mechanism is proposed that an antiviral
state should be established in the host cells by activating expression of a set of antiviral-relevant genes. In this study,
suppressive subtractive hybridization is applied to constructing a subtracted cDNA library with mRNAs isolated from UV-inactivated
GCHV infected and mock-infected CAB cells. 272 differential cDNA fragments are identified by both PCR and dot blot from the
subtractive cDNA library. Sequencing analysis reveals 69 genes, including 46 known gene homologues, and 23 unknown putative
genes. The known genes include the genes involved in interferon signaling pathways, such as Stat1 and Jak1, the antiviral genes, such as Mx and Viperin, and a set of interferon-stimulated genes observed in mammalian cells. Most of the unknown putative genes contain AU-rich
element in their sequences. Differential expressions of these genes are further confirmed by virtual Northern blot and RT-PCR.
The data imply that UV-inactivated GCHV is not only able to induce production of interferon in the infected CAB cells, but
also leads to the expression of a series of antiviral-relevant genes or immune-relevant genes, and therefore reveals that
the signaling pathway of interferon system and antiviral mechanism in fish are similar to those in mammals. |
| |
Keywords: | antiviral-relevant genes immune-relevant genes grass carp hemorrhage virus (GCHV) interferon suppressive subtractive hybridization |
本文献已被 维普 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |
|