| 蓝藻光合基因psaC在酵母中的转化和表达 |
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| 引用本文： | 李斌,李涛,童雪梅,王东辉,赵进东,吴光耀.蓝藻光合基因psaC在酵母中的转化和表达[J].北京大学学报(自然科学版),2001,37(6):754-759. |
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| 作者姓名： | 李斌 李涛 童雪梅 王东辉 赵进东 吴光耀 |
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| 作者单位： | 北京大学生命科学学院, |
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| 摘 要： | 聚球蓝藻（Synechococcus sp.PCC 7002）的psaC是光系统I的FA/FB脱辅基蛋白基因，其分子量8.9kD蛋白中含有2个4Fe-4S中心，构建了psaC基因的表达载体pYES2-psaC，将表达载体转化到酵母（Saccharomyces cervisiae）中，Southern杂交证明psaC基因已被转化，诱导培养后的蛋白电泳显示psaC基因获得高效表达。
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| 关 键 词： | 聚球蓝藻 psaC 酵母 基因转化 光合基因 基因表达 表达载体 psaC蛋白 含铁量 |
Expression of Photosynthetic Gene psaC of Cyanobacteria in Saccharomyces cerevisiae |
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| LI Bin LI Tao TONG Xuemei WANG Donghui ZHAO Jindong WU Guangyao.Expression of Photosynthetic Gene psaC of Cyanobacteria in Saccharomyces cerevisiae[J].Acta Scientiarum Naturalium Universitatis Pekinensis,2001,37(6):754-759. |
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| Authors: | LI Bin LI Tao TONG Xuemei WANG Donghui ZHAO Jindong WU Guangyao |
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| Abstract: | The psaC Gene of Synechococcus sp. PCC 7002 encodes the F A/F B aprotein of photosystem I,a protein with a molecular weight of 8.9 kD containing two 4Fe-4S centers.The expression vector of psaC gene was constructed,and transformed into Saccharoromyces cerevisiae.Southern blotting confirmed that psaC gene had been successfully transformed and Tris-Tricine-PAGE revealed that psaC gene had been highly expressed after incubating the transformed strains in the inducing culture. |
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| Keywords: | Cyanobacteria psaC Saccharomyces cerevisiae transformation |
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