| Two virus-encoded RNA silencing suppressors, P14 of Beet necrotic yellow vein virus and S6 of Rice black streak dwarf virus |
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| 作者姓名： | ZHANGLingdi WANGZhaohui WANGXianbing LIDawei HANChenggui ZHAIYafeng YUJialin |
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| 作者单位： | StateKeyLaboratoryforAgrobiotechnology,ChinaAgriculturalUniversity,Beijing100094,China |
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| 摘 要： | Functional analysis for gene silencing suppressor of P14 gene of Beet necrotic yellow vein virus and S6 gene of Rice black streak dwarf virus was carried out by agro- infiltration with recombinant vectors of Potato virus X. The phenotype observation of green fluorescent protein (GFP)expression and Northern blot showed that the gene silencing of gfp transgenic Nicotiana benthamiana induced by homologous sequence was strongly suppressed by the immixture infiltration of either the P14 or the $6. In the suppressed plants, the gfp mRNA accumulation was higher than that in the non-suppressed controls and the symptoms caused by PVX infection became more severe, especially the gfp DNA methylation of plant genome was significantly inhabited when co-infiltrated with RBSDV S6 gene. These results suggested that these two virus genes were potentially to encode for proteins as RNA silencing suppressors.
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| 关 键 词： | 抑制物 病毒 编码 S6 P14 甜菜 水稻黑条萎缩病 |
| 收稿时间： | 5 December 2004 |
Two virus-encoded RNA silencing suppressors, P14 ofBeet necrotic yellow vein virus and S6 ofRice black streak dwarf virus |
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| ZHANGLingdi WANGZhaohui WANGXianbing LIDawei HANChenggui ZHAIYafeng YUJialin.Two virus-encoded RNA silencing suppressors, P14 of Beet necrotic yellow vein virus and S6 of Rice black streak dwarf virus[J].Chinese Science Bulletin,2005,50(4):305-310. |
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| Authors: | Lingdi Zhang Zhaohui Wang Xianbing Wang Dawei Li Chenggui Han Yafeng Zhai Jialin Yu |
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| Institution: | (1) State Key Laboratory for Agrobiotechnology, China Agricultural University, 100094 Beijing, China |
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| Abstract: | Functional analysis for gene silencing suppres- sor of P14 gene of Beet necrotic yellow vein virus and S6 gene of Rice black streak dwarf virus was carried out by agro- in- filtration with recombinant vectors of Potato virus X. The phenotype observation of green fluorescent protein (GFP) expression and Northern blot showed that the gene silencing of gfp transgenic Nicotiana benthamiana induced by ho- mologous sequence was strongly suppressed by the immix- ture infiltration of either the P14 or the S6. In the suppressed plants, the gfp mRNA accumulation was higher than that in the non-suppressed controls and the symptoms caused by PVX infection became more severe, especially the gfp DNA methylation of plant genome was significantly inhabited when co-infiltrated with RBSDV S6 gene. These results sug- gested that these two virus genes were potentially to encode for proteins as RNA silencing suppressors. |
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