| 福氏志贺菌ipaC基因的克隆及序列分析 |
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| 引用本文： | 姚潇,王恒樑,杨伯伦,闫晓宇,史兆兴,冯尔玲,黄留玉.福氏志贺菌ipaC基因的克隆及序列分析[J].陕西师范大学学报,2003,31(2):89-91,94. |
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| 作者姓名： | 姚潇 王恒樑 杨伯伦 闫晓宇 史兆兴 冯尔玲 黄留玉 |
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| 作者单位： | [1]西安交通大学环境与化学工程学院，陕西西安710049；军事医学科学院生物工程研究所，北京100071 [2]军事医学科学院生物工程研究所，北京100071 [3]西安交通大学环境与化学工程学院，陕西西安710049 |
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| 基金项目： | 国家重点基础研究发展规划(973项目)资助项目(G1999054103) |
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| 摘 要： | 为了克隆福氏志贺氏菌的ipaC基因，以志贺氏菌福氏2a菌株为摸板进行PCR，并将PCR产物插入列pMD-T载体中，得到阳性重组子，并命名为pMD-ipaC．测序结果显示，其核苷酸序列与文献报道的序列有差异，但编码的氨基酸序列相同，表明志贺氏菌的ipaC基因已被成功克隆．
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| 关 键 词： | 细菌性痢疾 福氏志贺菌 ipaC基因 基因克隆 序列分析 致病机制 |
| 文章编号： | 1001-3857(2003)02-0089-03 |
Cloning and sequencing of ipaC gene from Shigella flexneri |
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| YAO Xiao ,WANG Heng-liang ,YANG Bo-lun ,YAN Xiao-yu ,SHI Zhao-xing ,FENG Er-ling ,HUANG Liu-yu.Cloning and sequencing of ipaC gene from Shigella flexneri[J].Journal of Shaanxi Normal University: Nat Sci Ed,2003,31(2):89-91,94. |
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| Authors: | YAO Xiao WANG Heng-liang YANG Bo-lun YAN Xiao-yu SHI Zhao-xing FENG Er-ling HUANG Liu-yu |
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| Institution: | YAO Xiao 1,2,WANG Heng-liang 2,YANG Bo-lun 1,YAN Xiao-yu 2,SHI Zhao-xing 2,FENG Er-ling 2,HUANG Liu-yu 2 |
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| Abstract: | To clone ipaC gene of Shigella flexneri , PCR is performed with the stains of S. flexneri 2a as template, and the PCR product is inserted into pMD-T vector. The positive recombinant is obtained and designated as pMD-ipaC. Sequencing results show that there are differences in nucleotide sequence with the reported, but the coded amino sequence is the same, indicating ipaC gene cloned successfully. This provides the basis for current understanding of the function of IpaC in the pathogenesis of S. flexneri further. |
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| Keywords: | Shigella flexneri ipaC gene cloning sequencing) |
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