Use of peroxidase-avidin conjugate for the demonstration of intracellular antigen |
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| Authors: | K Yasuda N Yamamoto S Yamashita |
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| Institution: | (1) Department of Anatomy, School of Medicine, Kitasato University, 228 Sagamihara, Kanagawa, (Japan);(2) Present address: Department of Anatomy, School of Medicine, Keio University, Shinanomachi, Shinjuku, Tokyo, Japan |
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| Abstract: | Summary Horseradish peroxidase was coupled to avidin by periodate oxidation. Using the peroxidase-avidin conjugate together with biotin-labeled anti-chymotrypsinogen antibody, chymotrypsinogen was clearly demonstrated in the zymogen granules of the pancreas. The reaction between avidin and biotin is so rapid and stable that the avidin-biotin system can be effectively applied to immunohistochemical studies.This work was supported by Grant-in-Aid for Scientific Research from The Ministry of Education, Science and Culture, The Japanese Government, No. 448086. |
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