| 质粒pBR322DNA与内切酶EcoRI相互作用的研究 |
| |
| 引用本文： | 张志勇,王艳伟,杨光参.质粒pBR322DNA与内切酶EcoRI相互作用的研究[J].温州大学学报(自然科学版),2011,32(5). |
| |
| 作者姓名： | 张志勇 王艳伟 杨光参 |
| |
| 作者单位： | 温州大学物理与电子信息工程学院,浙江温州,325035 |
| |
| 基金项目： | 国家重点基础研究项目(2007CB935900); 国家自然科学基金(10974146,20934004); 浙江省自然科学基金(Y6090222) |
| |
| 摘 要： | 借助原子力显微镜(AFM)对DNA与内切酶的切割与结合作用进行研究.当缓冲液中有Mg2+存在时,内切酶EcoRI会在单一识别位点处切割pBR322DNA,如果缓冲液中的Mg2+用Ca2+代替,内切酶EcoRI则会与pBR322DNA在单一识别位点处结合,并且切割率与结合率都随着内切酶浓度的增加而增加.
|
| 关 键 词： | DNA 内切酶 切割 结合 |
Study on Interaction between Plasmid pBR322DNA and Endonuclease EcoRI |
| |
| ZHANG Zhiyong,WANG Yanwei,YANG Guangcan.Study on Interaction between Plasmid pBR322DNA and Endonuclease EcoRI[J].Journal of Wenzhou University Natural Science,2011,32(5). |
| |
| Authors: | ZHANG Zhiyong WANG Yanwei YANG Guangcan |
| |
| Institution: | ZHANG Zhiyong,WANG Yanwei,YANG Guangcan(College of Physics and Electronic Information Engineering,Wenzhou University,Wenzhou,China,325035) |
| |
| Abstract: | The interaction of cleaving and binding between DNA and endonucleases EcoRI was observed by atomic force microscope(AFM).The findings showed that in the presence of Mg2+ in buffer,endonucleases EcoRI can cleave pBR322DNA on one specific recognition site;while,if Ca2+ took the place of Mg2+ in buffer,endonucleases EcoRI can bind pBR322DNA on the specific recognition site.The cleaving and binding ratio would increase with the increase of concentration of endonucleases. |
| |
| Keywords: | DNA Endonuclease Cleaving Binding |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
