灯盏花素对缺血性记忆障碍和血脑屏障的保护作用

采用行为观察和生化检测相结合的方法，研究了灯盏花素对小鼠缺血性记忆障碍和血脑屏障的保护作用．结果表明：前脑重复缺血再灌注使小鼠的学习记忆能力显著下降（P〈0．01），同时伴随脑内髓过氧化物酶（MPO）活性上升（P〈0.01）、脑组织伊文思蓝（EB）及水含量升高（P〈0.01）．灯盏花素治疗可提高缺血小鼠的学习记忆能力，并使脑组织EB含量下降（P〈0.05和P〈0.01）、水含量减少（P〈0.05），使血脑屏障损伤程度有所减轻；同时，伴随MPO活性的下降（P〈0.05和P〈0.01），炎症反应减弱．提示灯盏花素能减轻缺血再灌注引起的脑损伤，改善小鼠的学习记忆能力．该作用可能与灯盏花素提高脑组织抗血脑屏障损伤和抗炎症反应能力有关．

Protective effects of breviscapine on learning-memory dysfunction induced by ischemia-reperfusion in mice and its relationship to inflammation and blood-brain barrier damage

The protective effects of breviscapine on learning-memory dysfunction induced by ischemia-reperfusion in mice and its relationship to inflammation and blood-brain barrier damage were investigated through behavior observation and bio-chemistry analysis. The results were summarized as following： lschemia-reperfusion impaired learning-memory （ P 〈 0.05 and P 〈 0.01 ） , and increased the activity of MPO and the content of EB and water in mice （ P 〈 0.01 ）. It was found that breviscapine would make blood-brain barrier less serious and ameliorate neutrophil infiltration, remarkably decrease the content of EB （P 〈0.01） water （ P 〈 0.05 ） , and the activity of MPO （ P 〈0.05 and P 〈0.01） when compared to the model group, finally dramatically improve the ability showed an seemed to of learning-memory after ischemia-reperfusion （ P 〈 0. 05, P 〈 0. 01 ）. It implied that breviscapine improvement effect on the memory impairment in mice induced by ischemia/reperfusion, which be related with improvement of the anti-blood-brain-barrier-damage and anti-inflammation in brain