Function of resveratrol derived from transgenic plant expressing resveratrol synthase gene |
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Authors: | La Ping Cai Wenqi Zhang Jiuchun Zhang Fengli Fang Rongxiang |
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Institution: | (1) Laboratory of Plant Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, China |
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Abstract: | Two genes from grapevine coding for resveratrol synthase, named RS1 and RS2, were cloned by RT-PCR. AnEscherichia coli expression vector was constructed by insertion of RS1 into pBV221. A specific protein with the same molecular weight (42
ku) as the resveratrol synthase was expressed and used to prepare the rabbit antiserum. A plant expression vector was constructed
by inserting the RS1 gene into pBin438 downstream of the doubled CaMV 35S promoter and TMV-Ω fragment. PCR-positive transgenic
tobacco plants were obtained after transformation withAgrobacterium tumefaciens LBA4404 harboring the plant expression vector. Southern blot analysis demonstrated that the foreign gene was integrated into
the tobacco genome. The results of RT-PCR and Western blot indicated that the RS1 gene was transcribed and expressed. Formation
of resveratrol in transgenic tobacco was further determined by thin-layer chromatography of silica gel and HPLC. Increased
accumulation of human breast adenocarcinoma cells in G0 and G1 phases of cell cycle was observed in cells treated with resveratrol purified from transgenic tobacco as compared to the untreated
cells. |
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Keywords: | resveratrol synthase gene transgenic tobacco resveratrol human breast adenocarcinoma cell |
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