| ChsA启动子驱动的IPT基因植物表达载体的构建 |
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| 引用本文： | 邹金美,陈俊斌,徐惠娇,李灵灵,张颖.ChsA启动子驱动的IPT基因植物表达载体的构建[J].漳州师院学报,2010(1):105-109. |
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| 作者姓名： | 邹金美 陈俊斌 徐惠娇 李灵灵 张颖 |
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| 作者单位： | [1]漳州师范学院生物科学与技术系,福建漳州363000 [2]福建省漳浦县第四中学,福建漳浦363200 |
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| 基金项目： | 漳州师范学院大学生科技创新性项目（07xscxxsyxm48） |
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| 摘 要： | 为研究异戊烯腺苷类细胞分裂素与花衰老进程的相关性,本研究通过PCR方法从矮牵牛中克隆到花瓣特异性表达的矮牵牛查尔酮合成酶基因A（ChsA）的启动子PchsA,并以其取代植物表达载体pBI121中的组成型启动子CaMV35S,然后将IPT基因插入到PchsA启动子下游,测序结果显示花瓣特异性启动子驱动的IPT植物表达载体构建成功.
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| 关 键 词： | ChsA启动子 IPT基因 植物表达载体 |
The Construction of a Plant Expression Vector pBI121-PchsA-IPT Using ChsA Gene Promoter |
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| ZOU Jin-mei,CHEN Jun-bin,XU Hui-jiao,LI Ling-ling,ZHANG Ying.The Construction of a Plant Expression Vector pBI121-PchsA-IPT Using ChsA Gene Promoter[J].Journal of ZhangZhou Teachers College(Philosophy & Social Sciences),2010(1):105-109. |
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| Authors: | ZOU Jin-mei CHEN Jun-bin XU Hui-jiao LI Ling-ling ZHANG Ying |
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| Institution: | 1.Department of Biology Science and Technology,Zhangzhou Normal University,Zhangzhou,Fujian 363000,China;2.Zhangpu No.4 High School,Zhangpu,Fujian 363200,China) |
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| Abstract: | In order to further investigate the correlation between isopentenyl adenosine cytokinin and flowerage senescence development,we constructed the binary vector pBI121-PchsA-IPT.Firstly PchsA fragment was obtained from petunia hybrida genome DNA by PCR,then PchsA was used to substitute constitutive promoter CaMV35S in plant expression vector pBI121.Secondly,isopentenyl pyrophosphate transferase gene（IPT） was inserted into pBI121-PchsA using XbaⅠ and Sac Ⅰ restriction enzymes and T4 ligase.The sequence results showed that plant expression vector that flower petal specificity promoter PchsA drive IPT was constructed successfully. |
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| Keywords: | ChsA promoter IPT gene Plant expression vector |
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