| 功能基化聚丙烯酸甲酯固定青霉素G酰化酶的性质 |
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| 引用本文： | 周艳芬,潘延云,张贺迎,王书香,武金霞. 功能基化聚丙烯酸甲酯固定青霉素G酰化酶的性质[J]. 河北大学学报(自然科学版), 2000, 20(3): 271-274 |
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| 作者姓名： | 周艳芬 潘延云 张贺迎 王书香 武金霞 |
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| 作者单位： | 1. 河北大学,生命科学学院,河北,保定,071002
2. 河北大学,化学系,河北,保定,071002 |
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| 基金项目： | 河北省教育厅科研项目，97301， |
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| 摘 要： | 采用肽键法将青霉素G酰化酶固定在功能化的聚丙烯酸甲酯上得固定化酶 ,其最适pH为8.2,最适反应温度为70℃ ,该固定化酶在pH6～10和40℃以下非常稳定 ,表观米氏常数为1.48×10-2mol/L,最大反应速度为5.09mmol/s.33℃下水解质量分数为5 %的青霉素G,使用63次,酶活力保留79.4 %.在4℃的湿润状态下贮藏25d,酶活力保留97.6 %.
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| 关 键 词： | 功能基化聚丙烯酸甲酯 青霉素G酰化酶 固定化酶 |
| 文章编号： | 1000-1565(2000)03-0271-04 |
| 修稿时间： | 2000-01-20 |
Properties of the Immobilized Penicillin Acylase by Functionalized Polymethyl Acrylate |
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| ZHOU Yan-fen,PAN Yan-yun,ZHANG He-ying,WANG Shu-xiang,WU Jin-xia. Properties of the Immobilized Penicillin Acylase by Functionalized Polymethyl Acrylate[J]. Journal of Hebei University (Natural Science Edition), 2000, 20(3): 271-274 |
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| Authors: | ZHOU Yan-fen PAN Yan-yun ZHANG He-ying WANG Shu-xiang WU Jin-xia |
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| Affiliation: | ZHOU Yan-fen,PAN Yan-yun,ZHANG He-ying(College of Life Science, Hebei University, Baoding 071002, China)WANG Shu-xiang(Department of Chemistry, Hebei University, Baoding 071002, China)WU Jin-xia(College of Life Science, Hebei University, Baoding 071002, China) |
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| Abstract: | The immobilized penicillin acylase from E.coli108 was made with functionalized polymethyl acrylate. The optimal pH was 8.2 and temperature was 70 ℃ for hydrolysis of penicillin G. The immobilized enzyme was stable in the pH range of 6.0～10.0, and at the temperature below 40 ℃. The apparent Michaelis Constant Km and v of the immobilized enzyme were 1.48×10-2 mol/L and 5.09 mmol/s. The remained activity was 79.6% and 97.6% after operating 63 times and after storage 25 days in wet. |
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| Keywords: | functionalized polymethyl acrylate penicillin acylase immobilized enzyme |
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