珍珠鸟和家鸡甲状旁腺激素3型受体(PTH3R)的结构与表达图谱分析

甲状旁腺激素(PTH)与动物钙稳态调控和骨代谢平衡相关,其生理作用是通过甲状旁腺激素受体(PTHR)介导。甲状旁腺激素受体家族包括三个不同的亚型,其中甲状旁腺激素3型受体(PTH3R)在非哺乳类脊椎动物生长发育过程中起着重要作用,然而PTH3R在鸟类中的研究则相对较少。 本研究采用RT-PCR方法,首先克隆了珍珠鸟和家鸡的PTH3R基因全长cDNA序列。结果显示,家鸡PTH3R (cPTH3R) cDNA全长1632 bp,编码543个氨基酸,珍珠鸟PTH3R(zPTH3R-w) cDNA序列全长1563 bp,编码520个氨基酸,其蛋白均含有信号肽序列、七次跨膜区等特征性结构。此外,在珍珠鸟中还发现一个新剪接变体zPTH3R-v1,其cDNA序列全长1468 bp,编码488个氨基酸,其缺失了第3外显子进而导致第1跨膜结构域缺失。利用生物信息学方法,我们还对珍珠鸟和家鸡PTH3R蛋白序列进行三维建模。 采用RT-PCR方法,本研究也对珍珠鸟PTH3R基因进行组织表达分析。结果显示,zPTH3R及其剪切变体zPTH3R-v1在珍珠鸟脑及外周组织中广泛表达。珍珠鸟和家鸡PTH3R基因的成功克隆与结构解析,将为下一步开展PTH3R在鸟类中的功能研究奠定重要基础。     

用路由软件组建BGP4+协议一致性测试平台

IPV 6协议是下一代互联网协议.BGP 4 协议是支持IPV 6的多协议扩展的外部网关路由选择协议.简要介绍了BGP 4 协议和路由软件Zebra.对BGP 4 协议一致性测试平台的结构进行讨论,给出了一个用路由软件Zebra组建支持IPV 6的BGP 4 协议一致性测试平台的例子.     

斑马鱼分枝杆菌病的研究概况

斑马鱼作为一种常用的实验模式生物,在生物学和医学的许多研究领域得到了广泛应用。然而,国内外对于其病害的研究极少。分枝杆菌病是实验斑马鱼的常见病,危害最大,约占其总病例数的20%。斑马鱼对分枝杆菌具有天然的敏感性,因此该菌对实验斑马鱼构成了巨大的威胁,影响了许多重要的科学实验。本文从病原的种类,生物学特性,疾病的症状,诊断方法和病原检测技术以及控制等方面对斑马鱼的分支杆菌病进行了介绍,希望能引起人们足够的重视,并能积极的展开相关研究。     

Do zebra stripes influence thermoregulation?

The temperatures of black and white stripes on two zebras and a zebra hide were measured, throughout separate sunny days in Kenya. There is a 12–15^oC difference between living zebras’ stripe temperatures throughout the middle seven daytime hours. The hide temperatures reach 16^oC higher than the living zebras. Like all equids, zebras sweat to keep cool. Movement of sweat away from the skin is accelerated by the recently discovered surfactant equid protein latherin, a vital component of cooling in racehorses. Latherin decreases the surface tension of the sweat, facilitating evaporative cooling at the hair tips. We suggest that the abrupt temperature difference between the stripes causes chaotic air movement above the hair surface, thus enhancing evaporative heat dissipation. This cooling mechanism explains the lower temperatures of living zebra stripes than those of the inanimate hide. We observed that the black stripes can be separately erected, while the white remain flat. This may further refine the mechanism. There is an ongoing debate about the function of zebra stripes, recently focussed on the fact that stripes deter biting flies. The data and observations in this paper suggest that the primary function of the stripes may be thermoregulation and a secondary benefit, fly-deterrence.     

钙化焙烧提钒废渣浸出毒性试验初步研究

对贵州省某矿业有限公司刚出炉的钙化焙烧提钒废渣进行浸出实验,并利用斑马鱼胚胎染毒实验探讨了钙化焙烧提钒废渣浸出液中重金属的联合毒性效应.实验结果表明:根据《固体废物浸出毒性浸出方法-水平振荡法》(HJ557-2010)试验规范进行浸出,钙化焙烧提钒废渣浸出液中V浓度符合《钒工业污染物排放标准》(GB26452-2011),其它7种重金属浓度均符合《污水综合排放标准》(GB8978-1996)中的排放限值;染毒后的斑马鱼胚胎没有表现出强烈的感应,孵化出的幼鱼表现正常.由此说明钙化焙烧提钒废渣浸出液毒性较小,不会表现出对环境的强烈污染效应.     

斑马粪便的沼气发酵潜力研究

在30℃恒温条件下,以斑马粪便为原料,采用批量发酵工艺,进行厌氧消化产沼气的实验。结果表明,斑马粪便的TS利用率是38.64%,VS利用率为45.44%.沼气发酵潜力为360mL/g.TS和550mL/g.VS.斑马粪便的沼气发酵潜力高于秸秆类原料,高于牛粪、猪粪、马粪、羊粪和人粪,低于鸡粪。     

Inhibition and sex specific induction of spawning by serotonergic ligands in the zebra musselDreissena polymorpha (Pallas)

Serotonin (5-hydroxytryptamine, 5-HT) stimulates spawning in the zebra mussel (Dressena polymorpha), a macrofouling European bivalve that has recently invaded North America. To develop methods of controlling zebra mussel spawning, two vertebrate serotonin antagonists, methiothepin and metergoline, known to bind with high affinity to snail 5-HT receptors, were tested for their ability to block 5-HT-induced spawning in zebra mussels. Methiothepin inhibited 5-HT-induced spawning at concentrations as low as 10^–6 M. Metergoline (10^–4 M) inhibited 5-HT-induced spawning; however, at lower concentrations (10^–8 to 10^–5 M), metergoline by itself significantly induced spawning in male, but not female zebra mussels. Metergoline (10^–5 M)-induced male spawning was inhibited by 10^–5 M methiothepin. Thus, methiothepin is the most effective inhibitor and metergoline the most powerful inducer of spawning yet tested in zebra mussels.     

斑马鱼GAPDH蛋白片段的原核表达差异分析

为了分析斑马鱼GAPDH基因不同区域原核表达构建的诱导效应差异,应用生物信息技术分析斑马鱼GAPDH分子结构,通过定向删除技术,构建表达部分斑马鱼GAPDH蛋白片段的质粒。通过IPTG诱导,SDS-PAG检测,分析GAPDH基因不同区域构建的蛋白表达差异。结果:引物对ZGCKF/ZGCXR构建的GAPDH重组表达质粒具有IPTG诱导表达效应,而引物对ZGNKF/ZGNXR构建的质粒则不具有IPTG诱导表达效应。因此,为了在原核表达真核蛋白时应考虑过表达的真核蛋白是否会影响宿主的代谢以及是否会导致宿主的抵抗,设计引物时应当选择合适的区域构建重组质粒才能获表达产物。     

基于二维泊松方程六阶紧致格式的多重网格方法

利用六阶紧致差分格式、结合多重网格V循环算法求解了二维泊松方程的Dirichlet边值问题，并用不同的松驰算子与四阶精度格式的多重网格方法进行了比较，计算结果表明，该方法在不明显增加计算量的前提下较四阶精度格式的多重网格方法具有更好的精确度和收敛阶，且ZLGS迭代不论对四阶精度还是对六阶精度格式的多重网格算法，都是一种较其他松弛算子更加有效的“光滑剂”。