MiR-205 inhibits the invasion and migration of esophageal squamous cell carcinoma by modulating SMAD1 expression

Esophageal squamous cell carcinoma （ESCC） is one of the most lethal cancers worldwide. In this study, we aimed to investigate the underlying mechanisms of metastasis inhibition by miR-205 in ESCC. In microRNA （miRNA） array and quantitative RT-PCR analyses, we found that the expression level of miR-205 was significantly lower in patients with lymph node metastasis compared with that in patients without lymph node metastasis. After transfection of miR-205 mimics or inhibitors into ESCC cell lines, a significant negative correlation was observed between the expression level of miR-205 and Smad 1. In luciferase reporter assays, we revealed that miR- 205 inhibited the expression of SMAD1 by targeting the 3＇ untranslated region （3＇-UTR） of SMAD1 mRNA in ESCC cells. Furthermore, our results showed that miR-205 sup- pressed the invasion and migration of ESCC cells, whereas Smadl increased their invasion and migration. Taken together, our study demonstrates that miR-205 functions as a suppressor of tumor metastasis by regulating SMAD1 expression through targeting the 3＇-UTR of SMAD1 mRNAin ESCC. Therefore, miR-205 may be a potential therapeutic target for miRNA-based therapy of ESCC.     

自然电位测井模拟实验及其研究

本文根据井下自然电场的成因、自然电位测井工艺过程以及模拟实验的相似理论,得到了部分空间物理模型的理论依据,按该理论设计制作了模拟实验装置,并进行了不同条件下的实验,最后得到了自然电位与地层电阻率、侵入半径、地层的水淹程度等因素的关系,还将实验结果与数值模拟和实测资料进行了对比分析。     

光纤网络入侵中的未感染节点检测

光纤网络采用开放性较强的分布式结构,易受到恶意数据和代码的入侵,提出基于多元节点属性分类的光纤网络入侵未感染节点检测算法研究。依据节点测距原理,提取光纤网络中全部节点的位置信息;选定与未感染节点类型相关的光纤节点特征属性,并针对节点属性和入侵类型建模;依据多元分类算法对提取的光纤节点样本空间采样特征数据进行学习和分类,识别出光纤网络中的未感染节点。仿真实验表明,提出的节点检测算法克服了传统算法的弊端和不足,能够有效降低通信成本和节点能耗、提高入侵检测率、延长光纤网络生命周期。     

外族入侵对英语习语的影响

英语是世界上词汇最丰富的语言之一,英语习语更是纷繁浩瀚。习语蕴涵着浓郁的西方文化气息,渗透着其他民族的优秀思想,展示着英语发展和演变的历史。本文主要通过英国历史上几次主要的古代战争,探究外族入侵对英语习语的影响。透过语言,了解历史。     

猫爪藤的物候观测

经过2002年～2004年研究，认为光强是影响猫爪藤开花结实的主要生态因子，初步摸清了猫爪藤的物候节律，并初步确定其人工清除的时间．每年7月上旬果实开始成熟，7月下旬-8月上旬果实大量成熟．然后蒴果开始开裂．猫爪藤的防除应该选在每年7月上旬种子成熟之前，否则待种子成熟后再采取措施，会人为增加种子的扩散和传播．     

广东农业外来有害生物空心莲子草入侵的历史阶段与特点

空心莲子草是广东的重要入侵植物之一,在广东的大部分地区均有分布,对广东的农业、水上交通、自然生态等产生较大的危害.文章对空心莲子草的历史阶段与特点进行研究可对其生长习性、入侵规律有更好的了解,有利于对其进行更好的防治与利用.空心莲子草在广东的入侵共分为三个历史阶段,第一阶段为引进与逸生时期（1970-1979年）,野生种群规模小、分散,对广东的地区的危害不大;第二阶段为建立种群与快速蔓延时期（1980-1999年）,入侵范围的不断增加,危害较大,莲草直胸跳甲对防治空心莲子草产生了一定防控作用;第三阶段为大规模危害与开发利用时期（2000年至今）,种群规模更大、危害的生境不断增加、危害范围进一步扩散,但防治技术并有一定程度的提高.     

Overexpression of PITPNM3 promotes hepatocellular carcinoma cell metastasis

A previous study indicated that C–C chemokine(C–C motif)ligand 18(CCL18)is capable of inducing tumor cell invasion and metastasis by interacting with receptor membrane-associated phosphatidylinositol transfer protein 3(PITPNM3)in breast cancer cells.The present study aims to investigate the correlation between the PITPNM3 expression and metastasis in hepatocellular carcinoma(HCC).Real-time quantitative polymerase chain reaction and Western blot were performed to detect the expression pattern of PITPNM3 in patient samples and HCC cell lines.Wound-healing and transwell chamber assays were performed to assess the migration and invasiveness of HCC cells,and the activation of the signaling protein downstream of PITPNM3 was also detected by Western blot and immunofluorescence.The results revealed that PITPNM3 was upregulated in HCC tissue compared to matched normal liver tissue.Silencing the expression of PITPNM3 by specific siRNAs markedly attenuated the invasive and metastatic abilities of HCC cells,whereas the upregulation of PITPNM3 significantly increased HCC cell mobility.Furthermore,inhibiting the expression of PITPNM3 suppressed the activation of Pyk2,FAK,and Src,while overexpression of PITPNM3enhanced the phosphorylation of FAK and Src in HCC cells.Besides,suppression of Pyk2 can also impair the clustering of integrin.These results imply that PITPNM3 is a vital determinant of HCC migration and invasion.     

略论辽宁满族的抗战特点

14年抗战中,辽宁满族在义勇军、人民革命军、抗日联军和东北军中同日本侵略者、日伪军进行了艰苦卓绝的斗争,付出巨大牺牲.国内外局势的变化,决定了辽宁满族抗战的鲜明特点,即由自发性转变为有组织性、群众性与广泛性及其斗争的复杂性、多样性和坚定性.探讨辽宁满族抗战特点对发扬中华民族不怕流血牺牲的爱国主义精神,鼓舞中华儿女为民族的伟大复兴,建设一个繁荣富强的和谐社会具有重大的现实意义.     

Juvenile Sphaeroma quadridentatum invading female-offspring groups of Sphaeroma terebrans

Female isopods Sphaeroma terebrans Bate 1866 are known to host their offspring in family burrows in aerial roots of the red mangrove Rhizophora mangle. During a study on the reproductive biology of S. terebrans in the Indian River Lagoon, Florida, USA, juvenile S. quadridentatum were found in family burrows of S. terebrans. Between September 1997 and August 1998, each month at least one female S. terebrans was found with juvenile S. quadridentatum in its burrow. The percentage of S. terebrans family burrows that contained juvenile S. quadridentatum was high during fall 1997, decreased during the winter, and reached high values again in late spring/early summer 1998, corresponding with the percentage of parental female S. terebrans (i.e. hosting their own juveniles). Most juvenile S. quadridentatum were found with parental female S. terebrans, but a few were also found with reproductive females that were not hosting their own offspring. Non-reproductive S. terebrans (single males, subadults, non-reproductive females) were never found with S. quadridentatum in their burrows. The numbers of S. quadridentatum found in burrows of S. terebrans ranged between one and eight individuals per burrow. No significant correlation between the number of juvenile S. quadridentatum and the numbers of juvenile S. terebrans in a family burrow existed. However, burrows with high numbers of juvenile S. quadridentatum often contained relatively few juvenile S. terebrans. The majority of juvenile S. quadridentatum found in family burrows of S. terebrans were smaller than the juvenile S. terebrans that were cared for by their mothers. The results indicate that the presence of S. quadridentatum in S. terebrans family burrows may negatively affect the duration of extended parental care in S. terebrans. It is not known why parental female S. terebrans are not able to discriminate against juvenile S. quadridentatum. Possibly, the fact that the two species are closely related facilitates S. quadridentatum sneaking into S. terebrans family burrows.     

防范ASP木马入侵虚拟主机的研究

随着网络技术和信息技术的发展,对于现有的网络资源和硬件设施,虚拟主机技术得到了充分的运用,是解决资源有限的最有效的方法之一。针对目前ASP木马入侵IIS虚拟主机的防范,提出了一些可行而实用的措施。