定量负荷对唾液溶菌酶含量影响初探

为探讨定量负荷对大学生唾液溶菌酶(lysozyme缩写LZM)含量的影响,我们对168名大学生LZM含量进行了测定分析。实验结果表明,在静态时,体育系学生LZM含量低于普系学生;定量负荷后即刻体育系学生LZM含量明显增加;不同运动专项的体育学生LZM含量亦有差别,球类专项学生其含量低于田径专项。     

对四苯硼化钠比浊法标准曲线线性关系的研究

用四苯硼化钠比浊法测钾时,加入甲醛和聚乙烯醇溶液,将使标准曲线的线性关系得到改善,从而提高了四苯硼化钠比浊法的精密度。     

艾氏卡试剂消化硫酸钡比浊法测定膨胀石墨中的硫含量

为了简便、准确、快速地测定膨胀石墨中的硫含量,建立了用艾氏卡试剂消化样品、硫酸钡比浊法测定硫含量的方法.实验确定艾氏卡试剂与膨胀石墨最佳物质的量比为1:15,焙烧温度为850℃;比浊法中测试液的pH=1.0,质量分数为5%的聚乙烯醇分散剂和无水乙醇助稳定荆的用量分别为2.0 mL和3.0 mL;稳定10 min后,在460 nm下和0.1～5.0 mg内,硫酸钡的吸光度符合朗伯-比尔定律;方法平均回收率分别为101.2%(ZnS)和99.5%(Na2SO4),相对标准偏差均不超过3.0%.方法操作简便、准确,能快速测定膨胀石墨中的硫含量.     

比浊法测定植物中微量硫

研究了在酸性溶液中有保护剂存在条件下，ＳＯ与Ｂａ形成均匀的ＢａＳＯ＿４微粒悬浮于溶液中，其最大吸收峰波长为４８０ｎｍ，反应酸度ｐＨ＝１．１～１．５，ＳＯ含量在０～２０μｇ／ｍｌ范围内服从朗伯－比耳定律，植物中大部份常见阳离子不干扰，操作简便、快迅，能达到满意的测定效果。     

Real-time models of morphogenetic processes

Summary Morphological transformations are often describable by simple series kinetic models,AB, ABC, etc., which allow assessment of the rates of interconversion of the distinguishable shapes or forms present and their probabilities of occurrence at various points in time, thus providing a means for kinetic comparisons with biochemical measurements of the molecular-level reactions that cause the transformations. When changes in cell morphology are followed turbidimetrically, the real-time progress curves can be simulated by fitting the data to a form of Beer's law for scattering by mixtures in which the species concentrations change with time in accordance with the chosen kinetic scheme. Because many even relatively large cells are mostly water, classical light scattering theory can be used to interpret the turbidimetric data in terms of simple geometrical models ofaverage cell size and shape suggested by microscopic examination. Two examples are briefly considered, the stimulus-induced changes in blood platelet shape and apparent size and their correlation with cytosolic-free calcium, and apparent swimming motion exhibited by neutrophils in suspension.What do you know about this business? the King said to Alice. Nothing, said Alice. Nothing whatever? persisted the King. Nothing whatever, said Alice. That's very important, the King said. Lewis Carroll (1865), in Alice's Adventures in Wonderland     

硫酸钡比浊法的改良及其在生物冶金浸出体系硫酸根浓度测定中的应用

为改良现有硫酸钡比浊法,以适应生物冶金复杂样品浸出体系中硫酸根离子浓度的测定,探讨不同稳定剂、无水乙醇用量、Tween-80用量和酸度等对硫酸钡分散体系稳定性的影响,得到比浊法测定硫酸根离子浓度体系中的最佳条件是:无水乙醇3.0 mL,0.1 g/L Tween 1.0 mL,pH=1.0.在测量波长420 nm处,硫酸根浓度在0.1～0.8mmol/L范围内符合比尔定律.该方法的检出限为0.55 mg/L,定量限为1.82 mg/L.该方法简便快速,回收率为98.9％～101.9％,相对标准偏差为0.68％～1.90％.与GB/T 5750.5-2006国标法比较,该方法操作简便、快捷,结果可靠,在生物浸矿液硫酸根离子浓度的测定中得到较好结果.     

共振光散射比浊法测定胶片废水中银离子的研究

氯化银胶体在375、496和565 nm产生3个明显的共振散射峰,其中496 nm处的散射峰强度最大且最稳定,适于进行共振光散射比浊法分析.探讨了共振光散射比浊法的机理,建立了在酸性氯化钠-乙二醇溶液中,共振光散射比浊法测定银离子的新方法.线性回归方程IRLS=7.60cAg+-4.82,R=0.999 8;检出限0.04μg/mL.此方法用于实际样品测定,取得了满意效果.     

共振光散射比浊法测定环境水样中Pb(Ⅱ)

基于铅与重铬酸钾反应产生沉淀拟定了一种测定铅的共振光散射比浊法.通过实验确定了适宜的反应条件.实验表明,在λ=497 nm处,该体系有较大且较稳定的共振光散射强度,并且共振光散射强度与铅浓度成线性关系,线性范围为0.1～1.0μg/mL,检测限为0.0573μg/mL.该方法快速,灵敏,简便.将该方法用于实际含铅水样中的铅的测定,并与原子吸收光谱法比较,结果较为满意.     

应用比浊法测定凝血酶样酶活性

传统常用目测法观察血浆凝固时间来测定凝血酶样酶的活性,因无法微观地检测凝固过程的起始而导致灵敏度低．应用比浊法检测凝血酶样酶活性,以东菱克栓酶为标准凝血酶样酶,在血浆凝固过程中,浊度随时间基本呈直线变化直至浊度变化最终趋于恒定,表明此时血浆已完全凝固．酶活与浊度一时间变化曲线的直线斜率之间存在严格的线性关系．线性范围为０．０５～０．６５ｕ／ｍＬ．结果表明,比浊法是一种改进的凝血酶样酶测活方法．     

A basic protein from bovine brain that co-precipitates with tubulin in vitro

A 193 kDa protein consisting of 58 kDa subunits, which has pI values of 8.50 and 8.65, was purified from bovine brain cytosol. It formed heavy precipitates with tubulin, and the molar ratio of tubulin dimer to this protein in the precipitate was 3.2. In contrast to microtubules containing ordinary microtubule-associated proteins, these complexes remained stable against cold and 1 mM CaCl₂.Acknowledgments. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.