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Johansson S Gullbo J Lindholm P Ek B Thunberg E Samuelsson G Larsson R Bohlin L Claeson P 《Cellular and molecular life sciences : CMLS》2003,60(1):165-175
Four novel proteins (phoratoxins C–F) have been isolated from the North American mistletoe Phoradendron tomentosum. The amino acid sequences of these phoratoxins were determined unambiguously using a combination of Edman degradation and
trypsin enzymatic digestion, and by electrospray ionization tandem mass spectrometry sequencing. Phoratoxins C, E and F consist
of 46 amino acid residues; and phoratoxin D of 41. All proteins had six cysteines, similar to the earlier described phoratoxins
A and B, which are thionins. The cytotoxicity of each protein was evaluated in a human cell line panel that represented several
cytotoxic drug-resistance mechanisms. For the half-maximal inhibitory concentrations (IC50 values) of the different cell lines in the panel, correlation with those of standard drugs was low. The most potent cytotoxic
phoratoxin C was further tested on primary cultures of human tumor cells from patients. The solid tumor samples from breast
cancer cells were 18 times more sensitive to phoratoxin C than the tested hematological tumor samples.
Received 30 September 2002; received after revision 28 October 2002; accepted 7 November 2002
RID="*"
ID="*"Corresponding author. 相似文献
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Stec B 《Cellular and molecular life sciences : CMLS》2006,63(12):1370-1385
Thionins belong to a rapidly growing family of biologically active peptides in the plant kingdom. Thionins are small (∼5 kDA),
cysteine-rich peptides with toxic and antimicrobial properties. They show a broad cellular toxicity against wide range of
organisms and eukaryotic cell lines; while possessing some selectivity. Thionins are believed to be involved in protection
against plant pathogens, including bacteria and fungi, by working directly at the membrane. The direct mechanism of action
is still surrounded by controversy. Here the results of structural studies are reviewed and confronted with recent results
of biophysical studies aimed at defining the function of thionins. The proposed toxicity mechanisms are reviewed and the attempt
to reconcile competing hypotheses with a wealth of structural and functional studies is made.
Received 3 December 2005; received after revision 6 February 2006; accepted 18 March 2006 相似文献
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