噬菌体展示禽流感病毒抗原变异性基因片段文库的构建

构建T7噬菌体展示禽流感病毒抗原变异性基因片段文库. 首先， 从Gene Bank中查找筛选禽流感病毒抗原变异性基因, 将其截短、 修饰、 简并后得到禽流感病毒抗原变异性基因微阵列. 其次， 将合成的禽流感病毒抗原变异性基因片段文库扩增、 酶切, 链接到双酶切后的T7噬菌体载体基因上, 构成重组噬菌体DNA. 最后， 重组噬菌体DNA经体外包装和扩增, 得到T7噬菌体展示文库, 并进行T7噬菌体展示文库滴度、 重组率和免疫活性测定. 实验结果表明, 从Gene Bank中查找、 筛选、 剪切和修饰共获得96 258条序列构建T7噬菌体展示文库, 原始文库滴度为3.6×107个菌落/mL, 重组率大于90%. 用禽流感病毒H5N1抗体进行捕获, 经聚合酶链式反应(PCR)鉴定, 得到理想目的条带, 证明噬菌体表面展示蛋白具有抗原活性, 可用于禽流感病毒感染患者的快速检测及抗原表位筛选.     

半刚性基层施工弯沉检测标准研究

根据重庆地区大量实测资料 ,对影响半刚性基层弯沉变异性的各主要因素进行了分析 ,并根据实际需要 ,对施工期实测弯沉的龄期与养生温度的影响进行了修正 ,得出可用于施工检测的弯沉控制标准     

A local positive feedback of the tropical Pacific ocean-atmosphere system on interdecadal timescales

SINCE THE 1990S, THE CLIMATIC VARIABILITY ON INTERDE- CADAL TIME SCALES BECAME THE FOCUS OF THE INTERNATIONAL CLIMATOLOGY RESEARCH MISSIONS[1―3]. ON TIME SCALES OF A DECADE OR MORE, THE OCEAN CIRCULATION PREDOMINATEDHEAT BALANCE AND HYDROLOGICAL CYCLE, S…     

Temporal-spatial dynamics of distribution patterns of microorganism relating to biological soil crusts in the Gurbantunggut Desert

Biological soil crusts serve as an important biological factor contributing to the sand fixation. This study was conducted to investigate the temporal-spatial variability of microorganism in crusts relating to locations, soil layers of sand dunes and seasons. At moss-dominated inter-dune areas, higher soil nutrient and water concentrations were likely to maintain the microbial activities. Bacteria showed the highest capabilities of settlement and growth in inter-dunes in both spring and autumn. Soil water content reached the highest value in soil crusts in the inter-dune areas, especially in spring. Variations of quantities of actinomyces and fungi basically showed the consistent trend in different locations of sand dunes. With the deepening of soil layers, vertical distribution of quantities of each microorganism group showed different characteristics because environmental factors fluctuated in both spring and autumn. Among different microorganism groups, bacteria were predominant, actinomyces the next and fungi the least in both spring and autumn in all soil layers （0-20 cm）. The proportion of bacteria and soil water content were higher in spring than those in autumn in all soil layers （0-20 cm）. No consistent trends were found in actinomyces and fungi. The results showed that the quantities of microorganisms were significantly positive correlated with organic matter content, soil water content, total N, total P, available P, available K, pH, electrical conductivity, total salt content,catalase, urease, phosphatase and alkaline phosphatase.     

Variability of surface circulation in the South China Sea from satellite altimeter data

11-year satellite altimeter sea surface height （SSH） anomaly data from January 1993 to December 2003 are used to present the dominant spatial patterns and temporal variations of the South China Sea （SCS） surface circulation through Empirical Orthogonal Function （EOF） analysis. The first three EOF modes show the obvious seasonal variations of SSH in the SCS. EOF mode one is generally characterized by a basin-wide circulation. Mode two describes the double-cell basin scale circulation structure. The two cells were located off west of the Luzon Island and southeast of Vietnam, respectively. EOF mode three presents the mesoscale eddy structure in the western SCS, which develops into a strong cyclonic eddy rapidly from July to September. EOF mode one and mode three are also embedded with interannual signals, indicating that the SCS surface circulation variation is influenced by El Nino events prominently. The strong El Nino of 1997/98 obviously changed the SCS circulation structure. This study also shows that there existed a series of mesoscale eddies in the western SCS, and their temporal variation indicates intra-seasonal and interannual signals.     

时空数据模型研究原则与表达方法的探讨

时空数据模型的三个基本要素是空间位置、属性和时间．确定研究尺度范围内地理信息基元，合理表达时空关系和模型的层次体系是时空数据模型研究的基本原则．时空数据模型的表达方法主要有序列快照模型、基图修正模型、时空复合立方体模型、基于特征的时空数据模型和图谱数据模型．目前条件下，时空数据模型研究的重要意义在于，扩展传统地理信息系统的功能，实现时态地理信息系统(TGIS)的时序分析与规律预测．     

土壤有机质空间变异特征研究

以面积4．7hm^2的农田为研究区，采用网格法选取了92个观测点取样，测定0～15cm耕层土壤的有机质含量。结果表明，土壤有机质表现为强烈的空间自相关，有机质的空间变异性主要由结构性因素引起，其变异函数符合指数模型。     

1986-2007年中国耕地面积时空变化分析

根据统计数据,运用逐步回归方法分析了我国1986-2007年耕地面积的时空变化规律及影响因素.结果表明:中国耕地面积和人均耕地面积一直在减少,1999年以后减速加快.以1996年为界线,中国耕地面积从1980-1990年东部部分省份锐减、中西部大部分省份增加的格局转变为1996-2007年的西部锐减、中部次之,东部减速缓慢的格局.1986-1996年耕地面积减少的主要因素是城市人口和人口自然增长率,1996-2007年主要因素是第二产业产值、全国总户数,1986-2007年主要是城市人口、第二产业总产值、乡镇企业增加值及耕地总量平衡政策的发展.     

汾河流域降水量及蒸发量的空间变异性分析研究

借助于地质统计学方法对汾河流域的多年平均降雨量和蒸发量的空间变异性进行了分析,揭示了多年平均降水量和蒸发量的空间变异特征,并应用Kriging方法进行空间插值.分析结果表明,汾河流域多年平均降水量和蒸发量在不同的分割距离范围内有不同的空间相关性,多年平均降水量具有比较明显的地带性,蒸发量分布变化地带性不强.