Molecular cloning and nucleotide sequence of classical swine fever virus strain Shimen

According to the previously published CSFV sequences, 18 paris of partially overlapping primers which span the entire genome of CSFV strain Shimen were designed and synthesized. Each cDNA fragment of strain Shimen was amplified by RT-PCR method from the anticoagulant blood of strain Shimen infected pig. The PCR fragments were cloned into pGEM-T vector respectively and sequenced. The results show that we have obtained the nucleotide sequence of strain Shimen. The viral RNA consists of 12 297 nucleotides including noncoding regions of 373 and 227 bases at the 5′ and 3′ end, respectively, and a single large open reading frame spanning 11 697 nucleotides in the middle, which encodes an amino acid sequence of 3 989 residues with a calculated molecular weight of 437.6×10³. The precisely sequencing of 5′ and 3′ termini is undertaking. Supported by the National Pandeng Project Huang Qianhua: born in 1968. Graduate student     

四个生态因子对集约化养猪生产的影响

从环境温度、湿度、光照及空气四个方面详尽阐述了其对集约化养猪生产的作用和影响，提出一些改善猪生产环境的建议，对提高工厂化养猪的生产水平和生态效益具有一定的指导作用。     

引入青海的长白猪繁殖性状相关及通径分析

应用通径分析的方法研究了引入青海的长白猪断奶窝重与其他产仔、哺乳性状之间的关系,分析了直接效应和间接效应,揭示了繁殖性状间的真实关系。     

猪链球菌病与猪链球菌2型

猪链球菌2型引发猪链球菌病,不仅可致猪患败血症突然死亡,还可感染与猪密切接触皮肤有伤口的人群,并可致人死亡。2005年7月,我国四川爆发了猪链球菌病,经对疫区采取封锁、隔离、消毒以及及时确诊病人等措施,并紧急生产、迅速使用猪链球菌2型灭活疫苗后,该病的流行得到了有效的控制。介绍了猪链球菌病的病原、流行特点等,以及相应的防控措施。     

从猪"高热病"谈我国养殖业亟待解决的问题

就猪“高热病”发生、流行与研究现状进行了简要论述,并结合作者的临床实践与研究提出了几点思考。指出猪“高热病”疫情反映了疫病风险已成为我国养猪业发展之瓶颈,严重制约了畜牧业的发展,必须采取切实可行的政策措施,加大对新发疫病的研究与监控力度,强化防疫意识,改善养殖环境,控制污染,走可持续发展的道路。     

Candidate Multi-Peptide-Vaccine Against Classical Swine Fever Virus Induces Strong Antibody Response with Predefined Specificity

IntroductionClassical swine fever virus(CSFV) is a pestiviruswhich causes significantmortality and morbidity inpigs.An epidemic of CSFV in a high pig densityarea can result in devastating financial losses,because few infected pigs can be effectively cured.In many countries in Europe and Asia,classicalswine fever (CSF) is controlled by vaccinationwith commercially available vaccine strains,suchas the Chinese vaccine strain(C-strain,i.e.,hogcholera lapinized virus) orsome modified-live vir…     

碱提法回收猪骨蛋白质的探讨

对碱提法回收猪骨蛋白质工艺参数的研究表明，4种工艺参数对蛋白质得率均有不同程度的影响。其顺序为浸提液pH＞浸提时间＞沉降pH＞浸提温度。碱提法回收猪骨蛋白质的最高得率为25．7％，最佳工艺参数为浸提时间105min，浸提液pH12．0，浸提温度30℃和沉降pH5．0。     

Velocity and myosin phosphorylation transients in arterial smooth muscle: effects of agonist diffusion

Summary Transients in myoplasmic [Ca²⁺] and in phosphorylation of the 20,000 dalton light chain of myosin have been reported following stimulation of vascular smooth muscle by various agonists. Since these transients are rapid compared with the time required to attain a steady-state stress, agonist diffusion rates may be a significant limitation in activation. The purpose of this study was to estimate the effect of agonist diffusion rates on the time course of activation as assessed by mechanical measurements of stress development and isotonic shortening velocities and by determinations of the time course of myosin phosphorylation. The approach was to measure these parameters in K⁺-stimulated preparations of the swine carotid media of varying thicknesses and to estimate the theoretical contributions imposed by diffusion rates and the presence of a diffusion boundary layer surrounding the tissue. The results show that the time course of parameters which are tissue averages such as stiffness, active stress, and myosin phosphorylation is dominated by agonist diffusion rates. The sequence of events involved in excitation-contraction coupling including agonist actions on the cell membrane, Ca²⁺ release, activation of myosin light chain kinase, and cross-bridge phosphorylation appear to be very rapid events compared with stress development. Estimates of unloaded or lightly loaded shortening velocities which are not simple tissue averages appear to provide an imporoved estimate of activation rates.Supported by a grant from the National Institutes of Health 5-PO1-HL19242. K. E. Kamm was supported by a National Heart, Lung, and Blood Institute Research Service Award HL-05957.     

水葫芦与猪粪好氧厌氧交替堆肥特征研究

为探讨好氧厌氧交替条件下水葫芦与猪粪堆肥的特征,将水葫芦、猪粪与木屑以1．7：1．0：0．3（质量比）的比例混合均匀,进行为期56天交替式好氧厌氧堆肥化处理．试验研究了堆肥过程中温度（T）、含水率（Mc）、pH、挥发性脂肪酸（VFA）、C／N、水溶性铵态（NH4＋-N）、挥发性固体（vs）、总有机碳（TOC）、总氮（TN）和总磷（TP）的变化特征．结果表明,与堆肥原料相比,好氧厌氧交替堆肥化使堆料TOC、TN及vs分别下降了47．91％、25．00％及19．32％．经测定,56天后堆料腐殖质含量为6．52％,表明交替好氧厌氧堆肥化能同时实现水葫芦与猪粪的减量化与资源化．     

Cloning, sequencing and expression of a swine IgG H chain gene inE. coli

A DNA fragment about 1.5 kb has been isolated from spleen of adult Chinese swine by RT-PCR. The DNA fragment encodes immunoglobulin IgG H chain gene. Sequencing analysis showed that the DNA fragment is 1 425 bp long, complete CDS. The C region of the gene has been classified as Subclass Ig γ3, and is the same as reported by Sun et al., but V region of the present gene is 42 bp less by comparison. The gene has been ligated into expression vector pET-3b (NSEB)( - ). A protein about 52 ku has been expressed in E. coli with an expression level of about 21 % .