SARS研究进展

本文对SARS病原学、流行病学、预防和治疗的研究进展进行了综述。     

2006年7月3日宁阳县大暴雨过程分析

分析了2006年7月3日山东省宁阳县大暴雨的形成过程,对这次大暴雨的形成机制进行了探讨。     

地震作用下高层建筑多重调谐质量阻尼器控制优化设计

通过多重调谐质量阻尼器(MTMD)系统参数的可能组合，得到了5种MTMD模型．基于结构受按振型——MTMD系统的力学模型，建立了设置这5种MTMD时结构的传递函数和动力放大系数统一表达式．于是5种MTMD的优化准则可统一定义为结构最大动力放大系数的最小值的最小化．基于MTMD的优化结果即最优频率间隔、最优平均阻尼比和最优调谐频率比，利用Wilson—θ法对一22层高层钢结构—MTMD系统进行了时程分析．分析中所用地震波分别为：EL—Centro地震波，Taft地震波，天津地震波和上海地震波．进而评价了5种MTMD的地震反应控制效果和冲程情况．研究表明，在高层钢结构建筑的MTMD地震反应控制中宜优先考虑MTMD(Ⅰ)和MTMD(Ⅴ)模型．     

Protein Subcellular Localization Prediction and Genomic Polymorphism Analysis of the SARS Coronavirus

Introduction In March 2003, a novel coronavirus (CoV) was dis-covered in association with the outbreak of severe acute respiratory syndrome (SARS)[1-3]. The complete genome sequence of several SARS-CoV isolates was soon determined and characterized[4,5]. Comparison of variant SARS-CoV genome sequences has identified certain genetic signatures that can be used to trace sources of infection[6]. Vaccines are now being devel-oped and molecular modeling has suggested that modi-fied rhinovir…     

地震时作用于深水桥墩上的动水力及对桥墩动力响应的影响

基于势流体理论,针对一典型实体矩形截面桥墩,分析了不同水深时,动水对桥墩自振特性的影响.研究了不同水深和地震波类型等条件下,作用于桥墩上动水压力的分布规律及其合力作用点位置,并分析了动水对矩形截面桥墩在地震作用下动力响应的影响.结果表明,由于动水和结构相互作用的影响,矩形截面桥墩的自振周期、墩底弯矩及剪力的峰值会随着水深的增加而不断增大.动水使墩底弯矩和剪力分别最多增大了31%和50%,因此动水对桥墩动力反应的影响不可忽略.并且,不同类型地震波对作用于桥墩上的动水压力分布的影响也不相同.根据日本规范计算作用于矩形截面桥墩上的动水力的结果与本文的结果更为接近.     

重症急性胰腺炎病人外周血单个核细胞线粒体DNA拷贝数的意义

目的探讨重症急性胰腺炎患者外周血单个核细胞线粒体DNA拷贝数与患者病情严重程度、预后的相关性。方法分离研究对象（重症急性胰腺炎患者34名,健康对照组17名）外周血单个核细胞,采用荧光实时定量PCR检测各组PBMC线粒体DNA相对舍量,其与严重程度、预后的相关性。结果重症急性胰腺炎患者线粒体DNA含量较健康对照组明显降低（P〈0．05）。患者单个核细胞线粒体DNA拷贝教与APACHEⅡ评分呈负相关（r=-0．425）其中并发症组mtDNA拷贝数显著低于无并发症组（P〈0．05）。结论重症急性胰腺炎患者外周血单个核细胞中线粒体DNA含量显著下降,与患者APACHEⅡ评分呈负相关,且低水平的线粒体DNA拷贝数提示并发症发生率的升高,因此,线粒体DNA拷贝数可作为潜在评估患者病情危重程度及预后的指标,这一发现值得进一步大型,前瞻性的研究。     

强烈段塞流特征参数试验研究

为了寻求强烈段塞流特征参数的变化规律,在高4 m、内径50 mm的管线中利用双平行电导探针测试了强烈段塞流的持液率信号,并用互相关法对其进行了分析,得到了强烈段塞流的液塞速度、液塞长度随气、液相折算速度及下倾管倾角的变化规律。结果表明,当气相折算速度恒定时,随着液相折算速度的增大,液塞速度、液塞长度均线性增大;当液相折算速度恒定时,随着气相折算速度的增大,液塞速度线性增大,而液塞长度呈双曲型减小;当气、液相折算速度恒定时,随着下倾管倾角的增大,液塞速度、液塞长度都稍有增大。     

Fine three-dimensional P-wave velocity structure beneath the capital region and deep environment for the nucleation of strong earthquakes

A detailed 3-D P-wave velocity model of the crust and uppermost mantle under the capitol region is determined with a spatial resolution of 25 km in the horizontal direction and 4-17 km in depth. We used 48750 precise P-wave arrival time data from 2973 events of local crustal earthquakes, controlled seismic explosions and quarry blasts. These events were recorded by 123 seismic stations. The data are analyzed by using a 3-D seismic tomography method. Our tomographic model provides new information on the geological structure and complex seismotectonics of this region. Different patterns of velocity structures show up in the North China Basin, the Taihangshan and the Yanshan Mountainous areas. The velocity images of the upper crust reflect well the surface geological, topographic and lithological features. In the North China Basin, the depression and uplift areas are imaged as slow and fast velocity belts, respectively, which are oriented in NE-SW direction. The trend of velocity anomalies is the same as that of major structure and tectonics. Paleozoic strata and Pre-Cambrian basement rocks outcrop widely in the Taihangshan and Yanshan uplift areas, which exhibit strong and broad high-velocity anomalies in our tomographic images, while the Quaternary intermountain basins show up as small low-velocity anomalies. Most of large earthquakes, such as the 1976 Tangshan earthquake (M 7.8) and the 1679 Sanhe earthquake (M 8.0), generally occurred in high-velocity areas in the upper to middle crust. However, in the lower crust to the uppermost mantle under the source zones of the large earthquakes, low-velocity and high-conductivity anomalies exist, which are considered to be associated with fluids, just like the 1995 Kobe earthquake (M 7.2) and the 2001 Indian Bhuj earthquake (M 7.8). The fluids in the lower crust may cause the weakening of the seismogenic layer in the upper and middle crust and thus contribute to the initiation of the large crustal earthquakes.     

重组SARS冠状病毒S蛋白的原核表达研究

目的克隆表达SARS冠状病毒的主要结构蛋白(S蛋白)。方法合成SARS冠状病毒S蛋白特异性基因片断并克隆入pET32a原核表达载体,转化BL21菌,经IPTG诱导高效表达得到重组S蛋白,并通过W estern印迹对重组蛋白质进行鉴定。结果重组蛋白质经镍柱亲和层析得到了部分纯化,免疫动物后得到SARS病毒的多克隆抗体。结论经E lisa检测,表达的重组S蛋白基本具备检测病人血清中抗SARS病毒IgG和IgM的能力,可进一步用于S蛋白功能研究与SARS诊断试剂盒的研制。