一种新的配电网故障恢复算法及其改进

当配电网中某运行设备发生故障并切除后,会引起一些负荷失去供电,此时需要快速有效地寻找恢复供电路径。文中介绍的算法根据配电网的辐射状结构特征,把电源点和失去供电的负荷点看作“兴趣点”,把寻找供电路径转化为求解给定图的“兴趣树”,从而简化了问题。计算结果表明,经多方面的改进后,算法更加快速有效,在配电系统自动化高层软件开发中,有在线应用前景,本算法适用于负荷集中的１０ｋＶ以上配电网。     

鸡血超氧化物歧化酶脱辅,替代及重组的研究

本文对天然鸡血SOD中的金属辅基进行了去除和重组,初步研究了Cu~(2+)、Zn~(2+)、Co~(2+)等二价阳离子对SOD活性的影响。对天然态Cu_2Zn_2-SOD、重组Cu_2Co_2-SOD及脱辅E_2E_2-SOD的活性测定和光谱学行为的分析表明,重组Cu·Co-SOD具有同天然Cu·Zn-SOD类似的酶活性。在pH3.8时加入Cu~(2+)和Co(2+),重组的金属酶活性可恢复至天然酶活的85％,紫外吸收图谱也基本一致。     

招摇撞骗罪的重构探讨——兼论诈骗罪、冒充军人招摇撞骗罪的构建

在行为人冒充国家机关工作人员的身份或职称去骗取财物的情况下,刑法理论和司法实践却存在着很大的分歧,笔者认为要彻底解决这些矛盾,可以考虑对诈骗罪、招摇撞骗罪和冒充军人招摇撞骗罪在立法上进行重构。     

Nicotiana ovule extracts in duce nuclear reconstitution of demembranated Xenopus sperm in cell-free system

s Nicotiana tabaccum ovule extracts induced nuclear reconstitution of demembranated Xenopus leavis sperm in a ceil-free system. Demembranated Xenopus sperm began to swell after 15 rmin of incubation with Nicotiana ovulde extracts. Accompanying the process of incubation,Xenopus sperm decondensed and their shapes changed gradually from long and ellipse to round. The completely decondensed chromatin was surrounded with membrane structure, which was a mixture envelope of a double membrane and a single membrane. Nucleosome assembly was verified by means of micrococcal nuclease digestion to reconstituted nuclei and DNA electrophoresis. Nicotiana ovule extracts supplied one more experimental model and system.The new system could promote powerfully the research on mechanisms of cell division and cell cycle regulation.     

Nicotiana ovule extracts induce nuclear reconstitution of demembranated Xenopus sperm in cell-free system

Nicotiana tabaccum ovule extracts induced nuclear reconstitution of demembranated Xenopus leavis sperm in a cell-free system. Demembranated Xenopus sperm began to swell after 15 min of incubation with Nicotiana ovule extracts. Accompanying the process of incubation, Xenopus sperm decondensed and their shapes changed gradually from long and ellipse to round. The completely decondensed chromatin was surrounded with membrane structure, which was a mixture envelope of a double membrane and a single membrane. Nucleosome assembly was verified by means of micrococcal nuclease digestion to reconstituted nuclei and DNA electrophoresis. Nicotiana ovule extracts supplied one more experimental model and system. The new system could promote powerfully the research on mechanisms of cell division and cell cycle regulation.     

故障系统控制律重构的代数黎卡提方程方法

该文考虑故障系统的控制律重构问题，即重新设计控制律，使重构后的控制系统满足如下条件：（１）与重构前系统的误差（范数意义下）极小；（２）与重构前系统具有相同的稳定裕度；（３）控制能量极小。研究结果表明，上述综合指标约束下的控制律重构问题可转化为求解一代数黎卡提方程，并因此得到完整的解决。文中对一类代数黎卡提方程的求解算法做了相应的讨论，并提供了说明性的数值算例。理论分析及仿真结果都说明了该文所提设计     

Follicles were reconstituted from dissociated mouse fetal ovarian cellsin vitro

Early folliculogenesis involved in the interaction of germ cells and somatic cells is a complicated physiological event. Female germ cells are committed to differentiate into oocytes and finish complete development in the functional units of follicles. Thus there will be great significance in basal research and practices to evaluate the possibility of ovarian cells to reconstitute into follicles in vitro. In the present research, 12-16 dpc (days post coitum) mouse fetal ovarian cells were respectively isolated using collagenase digestion and cultured in droplets in vitro. The results revealed that the fetal ovarian cells of 12-16 dpc appeared to form multiple cell aggregates and tissue-like pieces in vitro. However, 12-13 dpc ovarian cells failed to form the follicles. 14-15 dpc ovarian cells were competent to form a few follicle-like complexes. Furthermore many small typical follicles were reconstituted from 16 dpc ovarian cells in vitro. The results showed for the first time that mouse embryonic ovarian cells were able to form the follicles in vitro. It was a gradual progression for the female germ cells to achieve the ability to induce somatic cells differentiation and reconstitu-tion into follicles, which may directly lead to the success in reorganization and transplantation of genetically modified ovary in vitro.     

蓝藻细菌光敏色素AphA（26-320）的体内重组与摇瓶发酵优化

将蓝藻PCC7120细菌光敏色素缺失突变体AphA（26-320）基因和血红素氧化酶（h01）及胆绿素还原（PcyA）的基因共同转化E．Coil BL21（DE3）,在IPTG的诱导下实现体内共同表达,进行体内重组,生成了具有可逆光致变色活性的AphA（26-320）-PCB,对重组茵的培养条件,诱导条件进行优化,确定适合合成AphA（26-320）-PCB的培养时间,诱导温度,摇床振荡速度等。