乙酰胆碱对人胃粘膜上皮细胞和胃腺癌细胞内Ca2+、Mg2+浓度的影响

目的:探讨乙酰胆碱对人胃粘膜上皮细胞和胃腺癌细胞内Ca2 、Mg2 浓度的影响。方法:利用大型生化分析仪,检测了乙酰胆碱作用后培养的人胃粘膜上皮细胞和胃腺癌细胞内Ca2 、Mg2 的浓度。结果:与正常胃粘膜上皮细胞比较,胃腺癌细胞内Ca2 、Mg2 浓度偏低(P>0 01)。在乙酰胆碱作用下,胃粘膜上皮细胞内Ca2 浓度升高(P<0 01),Mg2 浓度降低(P<0 01);胃腺癌细胞仅有Ca2 浓度升高(P<0 05),Mg2 浓度无变化。阿托品能阻断乙酰胆碱的作用(P<0 05)。结论:乙酰胆碱对胃粘膜上皮细胞和胃腺癌细胞的作用是通过毒蕈碱受体实现的。     

改善a-Si太阳电池界面特性的新方法

本文介绍一种用非晶金属硅化物改善a—Sipin太阳电池TCO/p界面特性的新方法.文章中详细讨论了载流子的传输模型并给出了一系列的实验数据.利用这种方法,用单反应室等离子体CVD装置使a—Si电池的短路电流提高了10%以上,并已获得10.6%的转换效率.     

Influence of Microstructure and Sintering Routes on Transport Properties of Apatite Materials for Fuel Cells

1 Results Oxy-apatite materials are thought as zirconia-substitutes in Solid Oxide Fuel Cells due to their fast ionic conduction. However, the well known difficulties related to their densification prevent them from being used as such. This study presents strategies to obtain oxy-apatite dense materials and the influence of elaboration route on transport properties. Particular emphasis is put on the microstructure effect on ion conduction. By the combined use of freeze-drying and conventional or spark p...     

New Membranes Based on Polybenzimidazole for Polymer Fuel Cells

1 Results Acid-doped polybenzimidazoles[1] are particularly appealing because of high proton conductivity with no or low humidification and promising fuel cells performances. PBI, in fact, contains basic functional groups which can easily interact with strong oxo-acids, such as H3PO4 and H2SO4. The acid partially protonates the polymer and partially is freely dispersed in the polymer backbone, so allowing proton migration via Grotthuss mechanism along the anionic chains[2]. Anyway, a technological limit...     

DEA-based anti-HIV immunotherapy model

Acquiredimmunitydeficiencysyndrome(AIDS)isoneofthemostfataldiseasesthathasspreadacrossthewholeworldsinceitfirstoccurred.Itiscausedbythehumanimmunodeficiencyvirus(HIV)[1].Mostofthechemotherapiesareaimedatkillingorhaltingthepathogen,butanewtreatmentistoreduceviralpopulationandimprovetheimmuneresponse.Thistreatmentcanboosttheimmunesystemandservetohelpthebodyfightinfectiononitsown,soitiscalledantiHIVimmunotherapy[2,3].TheaimofthisworkistofindefficientstrategiesforsuchtreatmentsusingtheDataEnv…     

塑料半薄切片法观察豆科植物根表面传递细胞

豆科植物合欢、紫云英、苜蓿等在接种根瘤菌后，部分根毛密集生长稍有膨大的根段，经FAA固定，GMA包埋，半薄切片（3um),PAS反应染色，在光学显微镜油镜下观察根的横切面发现，其表皮细胞或外皮层细胞的外切向壁上有明显的壁内突结构，属典型的根表面传递细胞，采用半薄切片，不仅操作简单，费用较少，且较易确定传递细胞发生的部位。     

Comparison of the effects of different isomers of bicuculline infused in the preoptic area on male rat sexual behavior

Summary Intracerebral infusion of (+) bicuculline methiodide, but not of its (–) isomer, in the preoptic area, stimulated masculine sexual behavior in rat as evidenced by a decrease in the number of intromissions preceding ejaculation and a shortening of the ejaculation latency and postejaculatory interval. Data suggest a role of the GABAergic system in mediating masculine sexual behavior.Acknowledgments. Authors wish to thank Ms Elisabeth Wallin for excellent technical assistance and Ms Madelene Kröning for preparing the figures.     

Aspirin-like drugs may block pain independently of prostaglandin synthesis inhibition

Summary Using flurbiprofen, a chiral anti-inflammatory and analgesic 2-arylpropionic acid derivative, the enantiomers of which are not converted to each other (less than 5%) in rats or man, we obtained evidence that prostaglandin synthesis inhibition is primarily mediating the anti-inflammatory activity but prostaglandin synthesis independent mechanisms contribute to the analgesic effects. Thus, the S-form inhibited prostaglandin synthesis, inflammation and nociception in rats. The R-form had much less effect on prostaglandin synthesis and did not affect inflammation. It did, however, block nociception in rats almost as potently as the S-form. S-flurbiprofen, in contrast to the R-form, was clearly ulcerogenic in the gastrointestinal mucosa. These results indicate additional molecular mechanisms of analgesia and suggest the use of R-arylpropionic acids as analgesics.     

慢性萎缩性胃炎与正常胃活检组织RNA分布的研究

采用异硫氰酸荧光素（FITC）标记的核糖核酸酶（FITC－RNase）作为探针，结合半薄切片技术，观察了慢性萎缩性胃炎与正常胃活检组织中RNA的分布变化，结果表明：慢性萎缩性胃啖腺体细胞内RNA明显减少，分布部位与正常胃腺体细胞差异较大，这些结果于慢性萎缩性胃炎的病理诊断提供了一些依据，对进一步从分子水平上研究慢性萎缩性胃炎的病理变化及其与胃癌的关系具有一定的意义。