Using of the surface plasmon resonance cytosensor for real-time and non-invasive monitoring of cellular effects in living C6 cells induced by PMA

By cell and molecular biology, the traditional life science research has been improved into modern ex-perimental science. Because cell is the basic structural and functional unit of the mysterious life, the study of life phenomena will be ultimately embodied into the study of cell biology. How to acquire a dynamic knowledge of the intricate biomolecular reactivity and process in living cells has become one of the hotspots in recent biological researches. For the complexity of cellular effects,…     

锑烯纳米结构等离激元的第一性原理研究

利用含时密度泛函理论(time-dependent density functional theory(TDDFT)),研究了锑烯纳米结构表面等离激元的激发特性,并给出了微扰场沿着扶手椅边界和Z字边界激发时锑烯纳米结构的吸收光谱.结果表明沿不同的方向激发,吸收光谱不同.距锑烯纳米结构表面0.9处的能量共振点的电荷密度分布表明,在低能共振区,等离激元共振属于键合二聚体的等离激元模式(BDP).     

表面等离子共振生物传感器测定人免疫球蛋白的抗原活性

目的:研究人免疫球蛋白质与羧甲基葡聚糖的氨基偶联及其与其他蛋白质的特异结合作用。方法:测定不同条件下人免疫球蛋白G(IgG)在羧甲基葡聚糖表面静电吸附的影响,并采用氨基偶联的方法,将其交联固定于葡聚糖表面,测定其与羊抗人IgG的特异结合。结果:pH及离子强度对蛋白质在羧甲基葡聚糖表面静电吸附有较大的影响,流体速度控制蛋白质的静电吸附动力学过程,并对其静电吸附量产生影响,其与羊抗人IgG有很强的特异结合作用,表现为快结合慢解离的动力学过程。结论:3.0相似文献   

表面等离子共振检测葡萄糖浓度的数据处理方法

根据表面等离子共振(SPR)检测葡萄糖浓度时信号和噪声的特点,研究了寻找曲线最低点算法和基线动态调整方法等SPR数据后处理方法.采用加权质心法精确地捕获到了表面等离子共振曲线的最低点,采用固定计算点数的动态基线法调整基线位置,消除了基线附近点值的波动对测量结果的影响.模拟仿真及实验验证结果表明,采用固定点数的动态基线法和加权质心法处理SPR数据,能显著提高SPR检测葡萄糖的分辨率,在葡萄糖浓度检测技术中有很好的应用前景.     

表面增强光谱和表面等离激元共振传感器

表面等离激元光子学是研究光和金属表面自由电子耦合所引起金属表面电荷密度振荡的性质及其应用的一门学科. 金属中的自由电子在入射光的作用下产生集体振荡. 在垂直表面的方向上强度呈指数衰减, 使得亚波长金属结构中光场高度局域. 由于独特的光学性质, 使得其具有广泛的应用, 其中两个重要的分支为: 表面增强光谱和表面等离激元共振传感器. 表面增强光谱传感器是利用纳米结构的巨大表面增强效应来直接探测表面分子,表面等离激元共振传感器通过检测目标分子对等离激元共振峰的影响进行定性定量检测.这两种优势互补的传感器技术都可以达到单细胞甚至单分子的检测水平. 本文将论述表面等离激元光子学的原理、表面增强光谱和表面增强光谱传感器研究领域的国内外最新进展和发展趋势.     

Application of surface plasmon resonance in screening adsorbents and explaining adsorption phenomena using model polymers

A surface plasmon resonance (SPR) technique was developed as an online screening method to identify effective adsorbents for the peptide NH₂-Cys-Thr-Trp-Trp-COOH (CW-4). To validate the SPR technique, several linear polymers containing amino acid residues were synthesized as models of their corresponding adsorbents. SPR screening of all the linear polymers demonstrated that the linear polymer containing phenylalanine residues (PPhe) exhibited the highest affinity for CW-4 among the polymers tested. In accordance with the screening results from SPR, the adsorbent containing phenylalanine (APhe) exhibited the highest adsorption capacity for CW-4. An interesting observation was that the amounts of CW-4 adsorbed on 3 adsorbents were quite small, even though both SPR and isothermal titration calorimetry (ITC) experiments indicated the presence of interactions between CW-4 and the three polymers. A kinetic analysis performed using SPR technology suggested such a phenomenon was ascribed to the poor stability of the CW-4/polymer complexes; that is, CW-4 associates with the polymers quickly, but it also dissociated quickly. The combined results suggested that SPR was a promising tool to identify the optimal adsorbent for peptides, analyze the interactions contributing to adsorption and explain adsorption phenomena using polymer models.     

Sensitivity dependence of surface plasmon resonance based sensors on prism refractive index

We theoretically and experimentally demonstrate that refractive index of the prism used to load metal film has significant influence on sensitivity of surface plasmon resonance based sensors. The prism with lower refractive index gives the sensors a higher sensitivity in detecting refractive index variations of a sample. We attribute this effect to the fact that a prism with low refractive index will increase coupling distance between surface plasmons and the medium under investigation. Foundation item: Supported by Wuhan University and National Education Ministry of China Biography: Wang Guo-ping (1964-), male, Professor, research direction: bolographic materials, diffractive optical elements, optical properties of metallic nanopracticles and metal-dielectric nanostructures     

硅基阻挡杂质带红外探测材料与器件研究进展

红外探测在天文物理研究领域具有极其重要的应用前景,由于天文深空探测对象的特殊性,对红外探测器的性能要求十分苛刻.阻挡杂质带(BIB)红外探测器,因其具有响应波段宽、噪声低、灵敏度高以及抗辐射性能好等优点,在过去30年中发展成为国际上天文领域首选的红外探测器.BIB红外探测器的探测波长覆盖中红外到远红外波段,它的推广应用促进人类在天文深空探测领域取得了一系列重大的科学成果.我国在BIB红外探测器的研究起步较晚,近年来也取得了较大进展,但是与国际领先水平相比尚有较大差距.本文主要回顾、评述了过去5年多我国在BIB红外探测器领域的研究进展,对BIB红外探测材料及其相应BIB器件的制备工艺、物性表征和光电响应特性,以及表面等离激元对器件光电响应的调控等做了详尽介绍,为我国BIB红外探测器的发展提供参考和实验依据.     

太赫兹等离子体激元增强传感研究进展

太赫兹作为一个大多数有机分子和生物大分子的振动和转动频率所在位置,已引起全社会的高度关注.近年来,随着太赫兹源和探测技术的不断发展更新,使得获取稳定的宽带太赫兹脉冲源成为一种常规技术.然而,相比于太赫兹波波长,由于分子的吸收截面非常小,导致微弱的相互作用,很难根据太赫兹特征光谱的变化对物质进行种类判定或定量分析的传感检测.如何增强太赫兹光谱技术的传感灵敏度,快速便捷地实现微量样品的检测,是太赫兹传感亟待解决的现实问题.电磁超表面凭借其独特的共振电场增强效应为解决这一问题提供了很好的解决方案.太赫兹等离子体激元增强传感研究自2000年起其发展已有了长足的进步,多种基于不同电磁超表面的太赫兹增强传感创新技术与方法被相继提出.本研究重点介绍这些针对太赫兹传感增强提出的表面等离子体共振传感器的最新研究进展,并详细指出它们的优势和不足.在此基础上总结了太赫兹增强传感的研究工作,并对其未来的发展方向进行展望.     

Plasmonic Bloch oscillations and resonant Zener tunneling in subwavelength metal-dielectric-air waveguide superlattices

In this paper,we study the propagation properties of surface plasmon polaritons in plasmonic single-cavity superlattices and two-cavity superlattices which are composed of two kinds of alternately stacked subwavelength metal-dielectric-air waveguides with large dispersion.Theoretical predictions of plasmonic time-resolved Bloch oscillations existing in single-cavity superlat-tices and resonant Zener tunneling(ZT) occurring in two-cavity superlattices by the transfer matrix method(TMM) are well dem-onstrated by the numerical simulations on the propagation of SPPs pulse in the two kinds of superlattices by the finite-difference time-domain(FDTD) method.The two proposed superlattices can be conveniently fabricated by present nanotechnology,and the study may promote the realization of plasmonic BO and resonant ZT in nanoscale devices experimentally.