Interacting domains of the HN and F Proteins of paramyxovirus

Binding sialates to hemagglutinin-neuraminidase （HN） activates （triggers） the fusion protein （F） to start the membrane fusion process of paramyxovirus, but the mechanism by which the HN and F associate with each other to induce membrane fusion is still unclear. It is noteworthy to study the interaction domains of HN and F of paramyxovirus. To screen interacting domains of the HN and F proteins of Avian parainfluenza virus-2 （APIV-2） and identify the structure of binding proteins, the GST pull-down assay and mass spectroscopy （MS） and circular dichroism （CD） experiments were performed in this study. The study revealed that the globular head region of HN protein tends to form a complex with either the heptad repeat 1 （HR1） or the heptad repeat 2 （HR2） of F protein respectively. This paper discusses the novel fusion mechanism induced by paramyxovirus HN and F proteins.     

人副流感病毒入侵宿主细胞的分子机制

综述了3型副流感病毒结合和进入宿主细胞的过程,重点讨论了受体结合分子血球凝集素-神经氨酶蛋白如何引发病毒与宿主细胞膜的融合过程,从分子水平上认识病毒入侵细胞的各个步骤,为进一步阻断病毒感染提供靶标。