罂粟碱、尼莫同治疗脑血管痉挛的疗效比较

通过动物实验，采用不同给药途径研究比较罂粟碱，尼莫同对脑血管痉挛的治疗效果。方法：采用枕大池注入自体动脉血建立兔ＣＶＳ动物模型，然后分成４组，分别采用经颈内动脉、周围动脉和静脉给予不同的药物，在ＳＡＨ形成前、形成后３ｄ及第１４ｄ分别测量 颈内动脉颅内段血流速度、颈内动脉颅内段管径，并在光镜下观察人动脉颅内段及大脑组织病理理变化。结果罂粟颈动脉内注射组的ＢＦＶ较罂粟碱静脉内、尼莫同动脉或静脉注射组的     

Enhanced reactivity towards flunarizine in cerebrovascular bed of spontaneously hypertensive rats

Summary Cerebral blood flow (CBF) was measured and cerebrovascular resistance (CVR) was calculated in anesthetized spontaneously hypertensive rats (SHR) and normotensive rats (NR) following the administration of incremental dosages of i.v. flunarizine or papaverine. CBF and CVR changes following papaverine were the same in both groups of rats irrespective of the dose of the drug. The effect of flunarizine was much more pronounced in SHR than in NR. The results point out the greater dependency of basal cerebrovascular tone in SHR upon Ca²⁺ influx into vascular smooth muscle cells.     

罂粟碱通过调节自噬对肝癌细胞放射敏感性的影响

为探究罂粟碱(Papaverine,PPV)和细胞自噬在原发性肝癌放射敏感性中的作用机理,将肝癌HepG2、Huh7细胞进行X射线照射,并将细胞分为阴性对照(NC)组、罂粟碱(PPV)组、单纯照射(IR)组，然后采用CCK8实验检测细胞的增殖情况,划痕实验检测细胞迁移情况,克隆形成实验检测细胞放射敏感性,实时定量PCR (RT-PCR)实验检测LC3B、ATG7 mRNA表达情况,蛋白免疫印迹(Western blot)实验检测自噬相关标志物LC3B、p62、p-PI3K/PI3K、p-AKT/AKT、p-mTOR/mTOR蛋白表达情况。研究结果显示,与NC组相比,罂粟碱对肝癌HepG2和Huh7细胞的增殖有明显的抑制作用(P<0.05),并可以抑制肝癌细胞的迁移能力(P<0.05)，显著降低肝癌细胞LC3B、ATG7 mRNA的表达水平(P<0.05)和LC3-Ⅱ/LC3-Ⅰ的蛋白表达,提高p62、p-PI3K、p-AKT和p-mTOR的蛋白表达。此外,罂粟碱还可以提高肝癌细胞的放射治疗(RT)效果。上述结果表明罂粟碱通过PI3K/AKT/mTOR通路抑制HepG2、Huh7细胞自噬,从而抑制肝癌细胞的增殖和迁移能力,并提高肝癌细胞放射敏感性。