四明湖水库翘嘴红鲌卵巢周年变化

本文根据四明湖水库翘嘴红鲌Erythroculter ilishaeformis Bleeker卵巢成熟度等级,成熟系数和周年组织学资料,对卵巢各成熟期中的卵母细胞形态结构及其演替进行了细胞学描述和探讨;判定该水库翘嘴红鲌的产卵期为6月下旬至9月上旬;成熟卵至少分两批产出,属分批产卵类型。     

牛卵母细胞的采集方法和卵巢贮存条件对其体外成熟的影响

比较了抽吸法和剖切法取得屠宰奶牛卵巢的可用卵母细胞数,并探讨了在卵巢运输过程中温度和时间对卵母细胞成熟率和卵裂率的影响.从屠宰场收集奶牛卵巢158个,用抽吸法抽取了96个卵巢,平均每个卵巢收集可用卵母细胞8.04枚(772枚/96卵巢);用切剖法切割了62个卵巢,平均每个卵巢收集可用卵母细胞18.89枚(1171枚/62卵巢).统计分析证明,两种采集方法获得的可用卵母细胞数差异极显著(p<0.01).将屠宰牛卵巢分别置于20℃～29℃和30℃～38℃的生理盐水中,在6h内收集到卵母细胞的成熟率和卵裂率分别为53.8%、31.7%和63.3%、34.3%,两者之间的差异均不显著(p>0.05).在30℃～38℃温度下,屠宰牛卵巢保存时间超过6h时,卵母细胞的成熟率和卵裂率显著下降,分别为36.1%和0%.与小于2h和2～6h相比差异极显著(p<0.01).     

Characterization of 17β-estradiol 3-(β-D-glucopyranoside) and 17-(α-D-glucopyranoside) as the metabolites of 17β-estradiol in the cultured ovaries of the silkworm,Bombyx mori

Summary The structures of the metabolites formed upon incubation of 17-estradiol with the ovaries of silkworm,Bombyx mori, have been determined as 17-estradiol 3-(-D-glucopyranoside) (1) and 17-(-D-glucopyranoside) (2) by spectroscopic means.     

Effect of continuous illumination on secretion of pregnane compounds by the rat ovary

Summary Differences in the secretion of pregnance compounds from rats with follicular polycystic ovaries under constant light and with normal preovulatory ovaries under light-dark conditions were compared. The injection of LH greatly increased the secretion of progesterone. 5-pregnane-3,20-dione and 3-hydroxy-5-pregnan-20-one, in both types of ovaries, but the response of the two progesterone metabolites in the polycystic ovaries was low, suggesting low 5-reductase activity.Acknowledgments. The authors thank the Endocrinology Study Section, NIADDK, Bethesda, MD, USA. for their gift of LH. This study was supported by Research Grant No. 466 154 from the Ministry of Education, Science, and Culture, Japan.     

遗传工程小鼠冻存卵巢异体原位移植比较研究

目的 对 C57BL / 6 J 为背景的遗传工程小鼠卵巢冷冻及复苏后原位移植进行研究,建立遗传工程小鼠卵巢 冷冻方法,弥补小鼠资源保种体系。 方法 采用 DAP213 方法冷冻保存了 C57BL / 6 J 及 SCARB2、PSGL1、IGB3S 三 个遗传工程小鼠 10 日龄幼鼠的卵巢,将四个品系 10 日龄幼鼠的新鲜卵巢及冻存复苏卵巢原位移植到 4 周龄 C57BL / 6 J 及 C57BL / 6 J( Cg) —Tyrc- 2 J / J( B6 albino)受体雌鼠,术后饲养 5 周与 10 周龄性成熟 C57BL / 6 J 雄鼠交 配,观察移植出生幼仔。 结果 C57BL / 6 J 新鲜卵巢和冻存卵巢,三个品系遗传工程小鼠的冻存卵巢原位移植到 C57BL / 6 J 和 B6 albino 受体中,均可以恢复正常功能,交配后得到仔鼠。 结论 采用 DAP213 方法冻存 C57BL / 6 J 及以 C57BL / 6 J 为背景的遗传工程小鼠卵巢,复苏后原位移植到 C57BL / 6 J 和 B6 albino,交配后产仔,卵巢冻存方 式是小鼠资源库保存的一个重要补充。     

哺乳动物卵巢的机能活动及其生理调控的研究

介绍了哺乳动物卵巢的机能活动和卵巢机能活动的激素调控机制。     

Makisterone A and 24-methylenecholesterol from the ovaries of the honey bee,Apis mellifera L

Summary Makisterone A, a 28-carbon ecdysteroid (molting hormone) has been isolated from the ovaries of queen bees. Analysis by reversed-phase and silica high performance liquid chromatography (HPLC) in conjunction with a radioimmune assay (RIA) revealed about 11 ng of makisterone A present per gram of ovaries on a fresh weight basis. No C₂₇ ecdysteroids were detected. The predominant neutral sterol present was 24-methylenecholesterol.