桑色素对小鼠T细胞增殖、周期和巨噬细胞分泌NO的影响

目的:研究桑色素(morin)对小鼠T细胞增殖、细胞周期和腹腔巨噬细胞分泌一氧化氮(nitrogen monoxidum,NO)的影响.方法:以佛波醇酯(phorbol 12,13-dibutyrate,PDB)和离子霉素(ionomycin,Ion)联合刺激淋巴结来源的小鼠T淋巴细胞,再以不同终浓度的morin与上述细胞共培养,利用流式细胞术(FCM),以羧基荧光素双醋酸盐琥珀酰脂(carboxyfluorescein diacetate succinimide ester,CFDA-SE)染色检测T细胞的增殖;以碘化丙锭(propidiumiodide,PI)染色分析T细胞的细胞周期;脂多糖(Lipopolysaccharide,LPS)刺激小鼠腹腔巨噬细胞,与不同浓度morin共培养,以Griess反应检测上清液中的NO含量.结果:CFDA-SE染色分析显示,PDB Ion组的增殖指数(proliferation index,PI)为1.70±0.05,各浓度的morin对PDB Ion刺激的T细胞增殖,具有明显地抑制作用,以100 μmol/L的morin抑制作用最明显,PI为1.03±0.01(P＜0.01).细胞周期的分析结果表明,morin组中处于S期的细胞比率较高,与PDB Ion组相比有明显差异.Griess反应检测NO含量的结果显示,各浓度组的morin均抑制LPS刺激巨噬细胞产生NO,100 μmol/L的morin可将LPS刺激巨噬细胞产生NO为(12.89±0.11)μmol/L降低为(7.25±0.05)μmol/L,抑制作用最强.结论:Morin可显著抑制PDB Ion刺激的T细胞增殖;其对增殖的抑制作用主要表现为S期的细胞的阻滞;对LPS刺激巨噬细胞产生的NO也有显著的抑制作用.     

铟-桑色素体系吸附溶出伏安法研究

对Ｉｎ（Ⅲ）－桑色素（ｍｏｒｉｎ）配合物在悬汞电极上的吸附伏安特性作了研究,确定了电极过程。在ＣｌＣＨ２ＣＯＯＨ底液中,有桑色素存在下,用１．５次微分吸附伏安法测定铟,线性范围和检测下限分别为３．５×１０－１０～１．５×１０－７ｍｏｌ／Ｌ和５×１０－１１ｍｏｌ／Ｌ。用此法测定了氢氧化铝、银粉和铅粉样品中的痕量铟,结果满意。     

钼－桑色素配合物的极谱波研究与应用

在ｐＨ２．１的硫酸－２．９６×１０－６ｍｏｌ／Ｌ桑色素－０．１７２ｍｏｌ／Ｌ氯酸钾底液中，钼在－０．４３Ｖ（ＳＣＥ）有一灵敏的极谱催化波，可用于测定微量钼．线性范围１．０４×１０－９～２．５０×１０－８ｍｏｌ／Ｌ，检出限１．８８×１０－１０ｍｏｌ／Ｌ．　极谱催化波法直接应用于三氧化钨光谱标样中微量相的测定，方法简便，准确可靠。     

不同羟基数目黄酮类药物与牛血清蛋白间相互作用

用荧光光谱法、分光光度法研究了3种黄酮类药物(染料木素、木犀草素和桑色素)与牛血清白蛋白(BSA)的相互结合作用.实验表明染料木素、木犀草素和桑色素与牛血清白蛋白的相互结合作用均为单一的静态猝灭过程,猝灭常数Ksv为4.22×104～4.83×105 L·mol-1,在溶液中结合作用强烈(结合常数KB为1.92×103～3.23×105 mol·L-1),形成稳定的配合物,求算了给体(BSA)与受体(黄酮类药物)作用距离r(3.35～4.80 nm).首次发现药物与BSA结合作用同羟基数目直接相关:随着药物分子中羟基数目(染料木素为3、木犀草素为4、桑色素为5)的增加,猝灭荧光峰位蓝移增大,作用距离r变小,结合作用逐渐加强;猝灭常数Ksv增大,更容易形成配合物;结合常数KB增大,形成的配合物更稳定.     

Standard molar enthalpy of formation of morin studied by rotating-bomb combustion calorimetry

Flavonols are plant pigments that are ubiquitous in nature. Morin and other related plant flavonols have come into recent prominence because of their usefulness as anticancer, anti-tumor, anti-AIDS, and other important therapeutic activities of significant potency and low systemic toxicity. The heat of combustion of morin (molecular formula, C₁₅H₁₀O₇·H₂O) in oxygen was measured by a rotating-bomb type combustion calorimeter, the standard molar enthalpy of combustion of morin at T = 298.15 K was determined to be Δ_c H _m^⦵ (C₁₅H₁₀O₇·H₂O, s) = −(5 937.99 ± 2.99) kJ · mol⁻¹. The derived standard molar enthalpy of the formation of morin in solid powder state at T = 298.15 K, Δ_f H _m^⦵ (C₁₅H₁₀O₇·H₂O, s), was −(1 682.12 ± 3.58) kJ · mol⁻¹, which provide an accurate data of the stability of morin to the pharmacy and pharmacology. Biography: HOU Hanna(1956–), female, Visiting researcher, Associate professor of Hubei University of Education, research direction: thermochemistry.     

Embodied Odysseys: Relics of stories about journeys through past,present, and future

This paper argues that the heritage represented by a museum should be seen not just in its individual objects but also in the relationships between them. The Conservatoire Nationale des Arts et Métiers and the Science Museum in London, the earliest great European science museums, were deeply concerned with the relationship between science and practice. The foundation speeches of the Deutsches Museum emphasised the concern with both past and future. Such ancestry provided hard-to-escape templates within which collections were built up over the subsequent century and more. Thus the Science Museum’s biotechnology collection developed at the very end of the twentieth century and albeit based on detailed historical research reflects the same concerns with science–technology relations as could be seen in the 1876 Loan Exhibition which started off the Science Museum. The physical presence of such carefully-structured accumulations of objects could embody and reinforce the reality of such categories as pure and applied science.     

在表面活性剂存在条件下用桑色素荧光光度法测定痕量铍

用荧光光度法研究了桑色素－Triton－X100－十二烷基硫酸钠－铍（Ⅱ）体系测定方法及条件在pH在12．5的H3BO3－NaOH缓冲溶液中，检测限为0．01μg／ml，铍的含量在0．1～1μg／ml范围内呈线性关系。本法灵敏度高、选择性好，可不加有机试剂萃取，在水相中直接进行测定合金及水样中痕量铍。结果令人满意。     

催化荧光光度法测定水中痕量锰(Ⅱ)

利用锰(Ⅱ)在氨性介质中催化H_2O_2氧化桑色素(morin)而使其荧光信号减弱为测定指示依据,介绍一种动力学荧光光度测定水中痕量锰(Ⅱ)的分析方法。检测下限为0.51ng/mL锰(Ⅱ)。对实际水样测定进行标准加入回收试验,回收率在93％～108％之间。