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Electrofusion of tobacco protoplasts in space 总被引：4，自引：0，他引：4
ZHENG Huiqiong WANG Liufa CHENG Aidi & LIU Chengxian Shanghai Institute of Plant Physiology Ecology Chinese Academy of Sciences Shanghai China Correspondence should be addressed to Zheng Huiqiong 《科学通报(英文版)》2003,48(18):1967-1970
Electrofusion of plant cell protoplasts is a techniqueto induce fusion between protoplasts by the reversibleelectric breakdown of their plasma membranes. This method is reliable and not toxic compared with chemicalfusion. Some somatic plant cell hybrids have been suc-cessfully produced by this method[1—5]. However, it is notwidely applied in agriculture due to the low yield of vi-able hybrids[6]. The reasons resulting in the low yield ofhybrids might be as the followings: (1) different densit… 相似文献
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