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1.
《科学通报(英文版)》2008,(19)
The purposes of this research were to study the stable expression of exogenous gene encoding therapeutic protein in attenuated Salmonella typhimurium, observe the metabolism of oral gene vaccine carried by attenuated Salmonella typhimurium in BALB/c mouse, and investigate the feasibility of prevention and treatment of tumors by the recombinant bacteria. Recombinant plasmid pcDNA3.1 VEGFR2(n1-7) was transformed into competent attenuated Salmonella typhimurium SL3261 to develop oral DNA vaccine SL3261-pcDNA3.1 VEGFR2(n1-7). To observe whether the exogenous gene can be expressed in the recombinant bacteria, PCR was performed to amplify the CMV promoter of the eukaryotic expression vector as the proof of stable expression of exogenous protein; transmission elec- tron microscopy (TEM) was applied to observe the morphology of the recombinant bacteria to confirm that the exogenous gene has no impact on the growth of the bacteria, and then BALB/c mice were immunized with the gene vaccine. After inoculation of the gene vaccine, the recombinant bacteria SL3261 could be detected in the tissues such as small intestine, colon, liver and spleen. And then, mice in each group were challenged with tumor cells. The results of animal experiment showed that tumor growth of the mice in experimental group was inhibited and survival time of immunized mice was prolonged compared with control groups. A higher lymphocyte infiltration in tumors from animals treated with DNA vaccine was observed. Immunohistochemical analysis of tumor samples revealed an en- hanced accumulation of CD8 cytotoxic T lymphocytes, as well as an increase in CD4 cells in the tumors of animals treated with the oral gene vaccine compared to tumors from control group mice. Ultrastructure of the tumor tissue showed that tumor cells in the samples of the immunized mice were well-differentiated. Our research confirmed that the exogenous gene can be stably expressed in the attenuated Salmonella typhimurium and has no impact on the growth of the recombinant bacteria; the exogenous gene can de delivered to the host by attenuated Salmonella typhimurium to produce anti-tumor effect with no obvious cytotoxity to the host. In this study, it is established that attenuated Salmonella typhimurium could be used as a vector for oral gene vaccine, and our study provided a theoretical basis for the body distribution and the metabolism of the recombinant bacteria. This strategy may provide a simple, safe and effective way for the prevention and treatment of tumors. 相似文献
2.
徐惠萍 《科技情报开发与经济》2007,17(36):18-20
分析了大学生产生心理疾病的原因,探讨了"阅读治疗"对大学生心理健康的作用,提出了高校图书馆开展阅读治疗的措施。 相似文献
3.
本文论述了描述微波与生物组织相互作用的数学表达式,讨论工作频率选择、透入深度、反射、极化、泄漏及防护等实际问题。 相似文献
4.
基于长期计划的动态给水管网优化设计模型 总被引:1,自引:0,他引:1
鉴于给水管网的优化设计的重要作用,提出了更符合实际、准确度更高的基于长期计划的动态给水管网优化设计模型。其目标函数中,动力费采用与时间相关的函数计算,折旧大修费采用与管段损坏率相关的函数计算,并建立了相应的动态优化设计模型。可以更精确地得出基于长期计划考虑管网优化设计结果。 相似文献
5.
FU Hongyuan 《系统科学与系统工程学报(英文版)》1994,(2)
ApplicationofMathematial-SimulatedModelSetupforEudcationalPlanningFUHongyuan(ChongqingInstituteofArchitectureandEngineering)A... 相似文献
6.
《科学通报(英文版)》1998,43(5):363-363
During the long period of time when people have been seeking for an effective therapeutic method for PD, the gene therapy has shown greater and greater advantages over other methods. It can be performed mainly in two ways: ex vivo and in vivo. With the former, TH gene as well as some neurotrophic factor genes (such as GDNF, BNDF genes) can be invited to some cell lines or primary cells thus forming engineered cells and then implanting them into brain. While with the latter, viral vectors including HSV-1, Ad, AAV that can be utilized to construct recombinant viruses, or non-virus vectors can be used to delived DNA into brain directly. The present review summarizes the recent research advances in the gene therapy for PD, and it is reasonable for us to predict a notable progress in prevention and treatment for PD in the next decade. 相似文献
7.
Ecological long-term effects of cultigens becoming feral and of naturalization of non-native species
Transgenic cultigens may become feral as we know of some non-transgenic cultigens. The article explains two basic ways how cultigens become feral: through hybridization with a closely related wild plant and through revert to the wild-type. A long list of examples of cultigens becoming feral in Central Europe is presented. The process of becoming feral is compared to the naturalization of non-native species (Exotic Species Model). Ecological long-term effects of both cultigens becoming feral and non-native species being naturalized are discussed with special regard to the predictability of such events. The ecological aspects discussed in the article are as significant for transgenic cultigens as for non-transgenic cultigens. 相似文献
8.
脱臭蒜素胶囊的研制 总被引:2,自引:0,他引:2
孙东 《科技情报开发与经济》2005,15(19):182-183
介绍了大蒜的主要化学成分及食疗价值,探讨了大蒜的脱臭机理,阐述了脱臭蒜素胶囊的制造技术. 相似文献
9.
HUANGShiwen ZHUORenxi 《科学通报(英文版)》2003,48(13):1304-1309
The gene delivery system is one of the three components of a gene medicine, which is the bottle neck of current gene therapy. Nonviral vectors offer advantages over the viral system of safety, ease of manufacturing, etc. As important nonviral vectors, polymer gene delivery systems have gained increasing attention and have begun to show increasing promising. In this review, the fundamental and recent progress of polymer-based gene delivery vectors is reviewed. 相似文献
10.
多曲方丝弓矫治技术的三维有限元分析 总被引:3,自引:0,他引:3
采用三维有限元法对后倾弯多曲方丝弓在四类牵引力作用下的矫治效果进行分析,在此基础上研究多曲方丝弓的整体力学特性,比较施加不同牵引力矫治的生物力学效果,揭示多曲方丝弓技术矫治特点。结果表明,无牵引力时,以第一、二前磨牙之间为界,多曲方丝弓矫治分别使前后牙向前后倾斜,且对前牙有压低作用;长Ⅲ类牵引时,对后牙的后倾作用有所增大,且传递力的性能比长Ⅱ类牵引好;Ⅱ类牵引力作用下的MEAW弓丝矫治对前牙有伸长作用,短Ⅱ类牵引对磨牙的伸长作用和对前牙的作用效果均小于长Ⅱ类牵引。因此,多曲方丝弓矫治技术有利于下颌Spee氏曲线的整平和牙齿的个别调控,对牵引力的传递作用强,同时对开拾错铪矫治有很好的效果。 相似文献