《启迪集》与日本医学之自立

顾名思义,"汉方"之意乃中国的医药之方。然二者之间又毕竟有所不同。若将"汉方"视为日本自身的传统医学知识体系,那么就必需思考与回答"自立"的标准为何,及其在何时开始走上独立发展之路的问题。实际上,这是一个不断发生的渐变过程,在此仅围绕后世派始祖曲直濑道三的医学著作《启迪集》,以及此前的田代三喜医学,其后的曲直濑玄朔、冈本玄冶、《众方规矩》及《古今方汇》等医学人物与著作进行研究,论说通常以为属于中国医学之后世派知识体系中的"日本化"要素。     

Nuclear science and technology in the Malaysian context: Three phases of technoscientific knowledge transfer (ETTLG)

This essay considers the development of the nuclear science programme in Malaysia from a transnational perspective by examining the interactions between state agents and other external nuclear-knowledge/technology related actors and agents. Going beyond the model of knowledge diffusion that brings together concerns articulated in Harris’s (2011) geographies of long distance knowledge and Reinhardt's (2011) role of the expert in knowledge transfer, the proposed three-phase model of knowledge transfer theorises the pathways undertaken by a late-blooming participant of modern science and technology as the latter moves from epistemic dependency to increasing independence despite the hurdles encountered, and the underdevelopment of many areas of its technoscientific economy. The model considers tensions stemming from the pressures of expediency for meeting national developmental goals on the one side, and the call to support the objectives of basic science on the other. The three phases of the model are epistemic transition, epistemic transplantation and localisation, and epistemic generation (ETTLG). As additional support for the proposed model, three arguments are proffered as deeper explanations of the epistemic goal by using Malaysia as a case study: knowledge transfer for political legitimization, knowledge transfer for countering agnotology, and knowledge transfer for social engineering and science diplomacy.     

Cloning and expression of the Chinese wheat mosaic virus RNA2 coat protein readthrough and 19 ku cysteinerich domains and localization of these proteins

The 5′-terminal (RTn) and 3′-terminal (RTc) halves of the coat protein readthrough domain and the 19 ku cysteine-rich protein of Chinese wheat mosaic virus (CWMV) were amplified by RT-PCR, cloned and expressed in E. coli. Antisera and monoclonal antibodies against these proteins were prepared by immunising these purified proteins to mice. Detection of RTn, RTc and 19 ku proteins in CWMV infected wheat sap and leaf tissue indicated that the RTn and RTc proteins were distributed on the surface of virus particles whereas the 19 ku protein was in the cytoplasm of the infected wheat cells.     

主从网络模式下中压配网电力线载波通信阻抗匹配方法

中压电力线信道环境恶劣,不同频点下信道输入阻抗存在较大差异,网络模式下的通信节点间的阻抗匹配具有重要意义。在中压配网电力线载波通信信道功率传输模型基础上,推导中压配网主从网络模式下从载波机间的阻抗匹配关系,提出一种基于差分定域的中压配网三点通信的阻抗匹配优化协调算法。该方法通过差分定域法界定寻优范围,在此基础上调整上游从载波机匹配阻抗,保证其接收功率电平大于最低可识别门限值的前提下,使下游从载波机接收功率最大。该协调优化方法可移植性强,优化速度快,相关算例验证了方法的正确性与有效性。     

A complex of Shc and Ran-GTPase localises to the cell nucleus

The three isoforms of the adaptor protein Shc play diverse roles in cell signalling. For example, the observation of p46 Shc in the nuclei of hepatocellular carcinoma cells suggests a function quite distinct from the better characterised cytoplasmic role. Ligands responsible for the transport of various Shc isoforms into organelles such as the nucleus have yet to be reported. To identify such ligands a far western approach was used to determine the p52 Shc interactome. The Ran-GTPase nuclear transport protein was identified and found to bind to p52 Shc in vitro with low micromolar affinity. Co-immunoprecipitation, pull down and fluorescence lifetime imaging microscopy experiments in stable cells confirmed cellular interaction and nuclear localisation. The nuclear transport factor protein NTF2, which functions in cohort with Ran, was shown to form a complex with both RAN and Shc, suggesting a mechanism for Shc entry into the nucleus as part of a tertiary complex. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. Received 20 October 2008; received after revision 04 December 2008; accepted 15 December 2008