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排序方式: 共有98条查询结果,搜索用时 15 毫秒
1.
海芋凝集素的理化性质鉴定 总被引:4,自引:0,他引:4
采用葡聚糖凝胶100(SephadexG-100)从天南星科植物海芋(Alocasiaodora(Roxb)C.Koch)中分离出了一种凝集素(简称AOL).AOL对兔血有较强的凝集效果,而对各类型人血的凝集活力较弱,在3种糖蛋白中,猪甲状腺球蛋白对AOL的凝血活力抑制最强;AOL对培养人外周血白细胞的转化率高达79%,超过对照PHA的转化率;用SephadexG-200柱测得AOL的相对分子质量为31500;用癌细胞体外培养的方法证明了AOL对肺腺癌细胞株P18具有较强的直接杀伤能力.此外还发现AOL对温度(80℃以下)、变性剂(盐酸胍和脲)有较高的稳定性,在pH6~10的范围内相当稳定. 相似文献
2.
野花生豆凝集素在pH7.0和pH5.0缓冲体系中,用EDTA去除其金属离子,CML凝血活性下降.Ca^2 、Mg^2 、Mn^2 、Zn^2 可使去除金属离子CML活性基本恢复到天然水平,表明这些二价金属离子为CML活性所必需.研究不同温度和不同浓度盐酸胍条件下CML的圆二色谱、荧光光谱及其凝血活性的变化,结果表明CML含有较多β-折叠,具有较强的抗变性能力;脲变性的时间扫描荧光光谱证实了CML变性过程的阶段性。 相似文献
3.
4.
采用酶标凝集素技术,在光镜水平对小鼠表皮发育期间蓖麻凝集素的受体进行定位,结果表明;在胚胎期间周皮细胞和角质层以及基膜中均有Gal残基分布;在表皮中间层,毛根原基处无Gal,在眼睑和眼睑缘处也无Gal分布,Gal可能参与表皮的角质化和基膜组建,与周皮在胚胎期发挥正常功能有关。 相似文献
5.
不同海藻凝集素对鲤免疫活性物的诱导作用 总被引:6,自引:0,他引:6
以条斑紫菜(Porphyra yezaensis)和裙带菜(Undaria pinnatifida)的凝集素作为免疫增强剂,来研究它对鲤免疫活性的诱导作用.实验结果表明,投喂和灌喂凝集素后,鲤血清中凝集素、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、溶菌酶活性与对照组相比都有提高,其中投喂条斑紫菜凝集素组比投喂对照组依次提高3.0倍、0.8倍、3.9倍、1.0倍;投喂裙带菜凝集素组比投喂对照组依次提高1.0倍、0.4倍、3.0倍、0.7倍,灌喂裙带菜凝集素组比灌喂对照组依次提高3.0倍、0.3倍、1.8倍;灌喂条斑紫菜凝集素组比灌喂对照组依次提高3.0倍、0.7倍.由此可看出:两种凝集素都可以作为一种免疫添加剂激活鲤的免疫系统,对鲤的非特异性免疫功能具有明显的增强作用.其中投喂条斑紫菜的效果更好. 相似文献
6.
Lehmann F Tiralongo E Tiralongo J 《Cellular and molecular life sciences : CMLS》2006,63(12):1331-1354
Sialic acids consist of a family of acidic ninecarbon sugars that are typically located at the terminal positions of a variety
of glycoconjugates. Naturally occurring sialic acids show an immense diversity of structure, and this reflects their involvement
in a variety of biologically important processes. One such process involves the direct participation of sialic acids in recognition
events through specific interactions with lectins, a family of proteins that recognise and bind sugars. This review will present
a detailed overview of our current knowledge regarding the occurrence, specificity and function of sialic acid-specific lectins,
particularly those that occur in viruses, bacteria and non-vertebrate eukaryotes.
Received 13 December 2005; received after revision 9 February 2006; accepted 15 February 2006 相似文献
7.
文蛤(Meretrix Meretrix)凝集素(MML)的初步研究——Ⅰ.MML在文蛤体内的分布及纯化 总被引:1,自引:0,他引:1
在文蛤(Meretrix Meretrix)体内具有血球凝集活性的凝集素(简称MML),经实验证实仅分布于文蛤外套腔液中。向外套腔液中加入固体硫酸铵达40%时,可使99%的MML沉淀。用不同饱和度的硫酸铵溶液依次对上述沉淀进行抽提,得到MML粗制品溶液,其比活比原始外套腔液提高了约9.95倍,总活力回收达48%。最后用自制的人血浆糖蛋白一Sepharose 4 B亲合层析柱可将上述MML粗制品的比活再提高400多倍。结果与原始外套腔液比较,MML终产品的比活总共提高了4313倍,总活力回收达44.8%,在电泳鉴定时显示出单一的区带。 相似文献
8.
Spiro RG 《Cellular and molecular life sciences : CMLS》2004,61(9):1025-1041
Misfolded or incompletely assembled multisubunit glycoproteins undergo endoplasmic reticulum-associated degradation (ERAD) regulated in large measure by their N-linked polymannose oligosaccharides. In this quality control system lectin interaction with Glc3Man9GlcNAc2 glycans after trimming with endoplasmic reticulum (ER) -glucosidases and -mannosidases sorts out persistently unfolded glycoproteins for N-deglycosylation and proteolytic degradation. Monoglucosylated (Glc1Man9GlcNAc2) glycoproteins take part in the calnexin/calreticulin glucosylation-deglucosylation cycle, while the Man8GlcNAc2 isomer B product of ER mannosidase I interacts with EDEM. Proteasomal degradation requires retrotranslocation into the cytosol through a Sec61 channel and deglycosylation by peptide: N-glycosidase (PNGase); in alternate models both PNGase and proteasomes may be either free in the cytosol or ER membrane-imbedded/attached. Numerous proteins appear to undergo nonproteasomal degradation in which deglycosylation and proteolysis take place in the ER lumen. The released free oligosaccharides (OS) are transported to the cytosol as OS-GlcNAc2 along with similar components produced by the hydrolytic action of the oligosaccharyltransferase, where they together with OS from the proteasomal pathway are trimmed to Man5GlcNAc1 by the action of cytosolic endo--N-acetylglucosaminidase and -mannosidase before entering the lysosomes. Some misfolded glycoproteins can recycle between the ER, intermediate and Golgi compartments, where they are further processed before ERAD. Moreover, properly folded glycoproteins with mannose-trimmed glycans can be deglucosylated in the Golgi by endomannosidase, thereby releasing calreticulin and permitting formation of complex OS. A number of regulatory controls have been described, including the glucosidase-glucosyltransferase shuttle, which controls the level of Glc3Man9GlcNAc2-P-P-Dol, and the unfolded protein response, which enhances synthesis of components of the quality control system.Received 26 January 2004; accepted 25 February 2004 相似文献
9.
10.
Diekman AB 《Cellular and molecular life sciences : CMLS》2003,60(2):298-308
Glycoconjugates in the mammalian reproductive tract are critical components of the molecular mechanisms that control sperm
maturation, sperm transport and gamete interactions. In the oviduct of many species, sperm transport and maturation are regulated
by protein-carbohydrate interactions that form a sperm reservoir. Subsequently, gamete interactions are mediated by the binding
of lectin-like sperm proteins with carbohydrate moieties on the zona pellucida. The sperm glycocalyx is extensively modified
during sperm transport and maturation. Multiple functions have been proposed for this dense carbohydrate layer overlying the
sperm plasmalemma, and sperm-surface carbohydrates have been implicated in immune-mediated human infertility. The structure
and function of glycoconjugates in the oviductal sperm reservoir, the zona pellucida, and on the sperm surface are reviewed. 相似文献